Qiu Jiamin, Wang Wanxia, Hu Shenqiang, Wang Yushi, Sun Wenqiang, Hu Jiwei, Gan Xiang, Wang Jiwen
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Comp Biochem Physiol B Biochem Mol Biol. 2018 Jul;221-222:29-43. doi: 10.1016/j.cbpb.2018.04.004. Epub 2018 Apr 21.
CCAAT/enhancer binding protein α, β, δ (C/EBP α, β, δ) are essential transcriptional factors in regulating adipose development. However, information about their sequence characteristics and functions during adipocyte development still remains scarce in birds. In present study, we found that duck C/EBP α, β, δ differed in their phosphorylation sites and low complexity regions (LCRs) among their orthologs and paralogs. Phylogenetic analysis showed that C/EBP α, β, δ had different evolutionary patterns, and each of duck C/EBP α, β, δ was strikingly diverged from orthologs of other Aves. Results of quantitative real-time PCR exhibited that C/EBP α, β, δ were all highly expressed in duck adipose tissues. Indeed, investigations of changes in both their mRNA levels and lipid droplet content during duck adipocytes differentiation showed that their expression profiles were closely related to cellular lipid accumulation. Furthermore, hierarchical clustering analysis of the C/EBPs and lipid metabolism-related genes expression profiles showed that C/EBP α was clustered with genes related to lipolysis, lipogenesis and fatty acid desaturation, whereas C/EBP β, δ were clustered with genes related to de novo lipogenesis and fatty acid elongation, which were different from mammals. In summary, C/EBP α, β, δ of duck differ from other species in their structures and have different effects on lipid metabolism during adipocytes differentiation. This research serve as a foundation for further investigations about avian C/EBP α, β, δ in adipocytes differentiation and adipose development.
CCAAT/增强子结合蛋白α、β、δ（C/EBPα、β、δ）是调节脂肪发育的重要转录因子。然而，在鸟类中，关于它们在脂肪细胞发育过程中的序列特征和功能的信息仍然很少。在本研究中，我们发现鸭的C/EBPα、β、δ在其直系同源物和旁系同源物之间的磷酸化位点和低复杂性区域（LCR）存在差异。系统发育分析表明，C/EBPα、β、δ具有不同的进化模式，鸭的C/EBPα、β、δ中的每一个都与其他鸟类的直系同源物有显著差异。实时定量PCR结果显示，C/EBPα、β、δ在鸭脂肪组织中均高表达。事实上，对鸭脂肪细胞分化过程中它们的mRNA水平和脂滴含量变化的研究表明，它们的表达谱与细胞脂质积累密切相关。此外，对C/EBPs和脂质代谢相关基因表达谱的层次聚类分析表明，C/EBPα与脂解、脂肪生成和脂肪酸去饱和相关基因聚类在一起，而C/EBPβ、δ与从头脂肪生成和脂肪酸延长相关基因聚类在一起，这与哺乳动物不同。总之，鸭的C/EBPα、β、δ在结构上与其他物种不同，并且在脂肪细胞分化过程中对脂质代谢有不同的影响。本研究为进一步研究鸟类C/EBPα、β、δ在脂肪细胞分化和脂肪发育中的作用奠定了基础。
