Volpato Andreia, Fortuoso Bruno F, Campigotto Gabriela, Glombowsky Patrícia, Bottari Nathieli B, Lopes Leandro S, Da Silva Aleksandro Schafer
Graduate Program of Animal Science, Universidade do Estado de Santa Catarina (UDESC), C, Chapecó, 89815-630, Chapecó, SC, Brazil.
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Rua Beloni Trombeta Zanin, Chapecó, 89815-630, Chapecó, SC, Brazil.
Exp Parasitol. 2018 Jun;189:16-18. doi: 10.1016/j.exppara.2018.04.008. Epub 2018 Apr 11.
The aim of this study was to verify whether secnidazole, given in a single oral dose (10 mg/kg), decreases or eliminates the excretion of Giardia duodenalis cysts. Holstein calves were raised from birth to 15 ± 2 days of age in individual stalls. Subsequently, 12 calves were grouped and housed in collective stalls. After seven days (day of life 21), we collected stool samples directly from the rectal ampulla in order to determine the degree of parasitic infection. Fecal examination was performed by a centrifugal-flotation technique, which allows for visualization and quantification of G. duodenalis cysts. After division into control and treatment groups, six animals were treated with one 400 mg secnidazole capsule. The first stool collection following treatment was performed on day 5 and the second on day 30. This experiment was repeated at 15 days, with a total of 24 calves studied. Animals on the farm where the experiment was conducted often suffer from giardiasis, despite hygiene care (disinfection) and adequate facilities. All 24 calves were excreting G. duodenalis cysts prior to starting treatment. Five days after receiving the treatment, animals in the experiment group were Giardia-negative, i.e., they did not excrete parasite cysts, whereas calves in the control group continued to excrete cysts. After 30 days of treatment, the stool of most treated animals (83.3%) remained free of G. duodenalis cysts. Therefore, we believe that secnidazole was 100% effective in eliminating the excretion of Giardia duodenalis cysts.
本研究的目的是验证单剂量口服塞克硝唑（10毫克/千克）是否能减少或消除十二指肠贾第鞭毛虫囊肿的排泄。荷斯坦犊牛从出生起在个体畜栏中饲养至15±2日龄。随后，将12头犊牛分组并饲养在集体畜栏中。7天后（即出生后第21天），我们直接从直肠壶腹采集粪便样本，以确定寄生虫感染程度。采用离心浮选技术进行粪便检查，该技术可对十二指肠贾第鞭毛虫囊肿进行可视化和定量分析。分为对照组和治疗组后，给6只动物服用一粒400毫克的塞克硝唑胶囊。治疗后的第一次粪便采集在第5天进行，第二次在第30天进行。该实验在15天后重复进行，共研究了24头犊牛。尽管进行了卫生护理（消毒）且设施充足，但进行实验的农场中的动物仍经常感染贾第虫病。所有24头犊牛在开始治疗前均排泄十二指肠贾第鞭毛虫囊肿。接受治疗5天后，实验组动物的贾第虫检测呈阴性，即它们不再排泄寄生虫囊肿，而对照组的犊牛继续排泄囊肿。治疗30天后，大多数接受治疗的动物（83.3%）的粪便中不再有十二指肠贾第鞭毛虫囊肿。因此，我们认为塞克硝唑在消除十二指肠贾第鞭毛虫囊肿排泄方面100%有效。
