de Aquino Monally Conceição Costa, Harvey Tatiani Vitor, Inácio Sandra Valéria, Nagata Walter Bertequini, Ferrari Elis Domingos, Oliveira Bruno César Miranda, Albuquerque George Rego, Widmer Giovanni, Meireles Marcelo Vasconcelos, Bresciani Katia Denise Saraiva
Universidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatuba, São Paulo, Brazil.
Universidade Estadual de Santa Cruz (UESC), Ilhéus, Bahia, Brazil.
Food Waterborne Parasitol. 2019 May 31;16:e00062. doi: 10.1016/j.fawpar.2019.e00062. eCollection 2019 Sep.
We performed molecular characterization of in buffalo calves from the Southwest region of São Paulo State, Brazil. A total of 183 fecal samples of Murrah breed buffaloes up to six months of age were collected. We examined these samples by the polymerase chain reaction (PCR) targeting the small-subunit ribosomal RNA gene and positive samples were characterized using additional PCR assays targeting a portion of the beta-giardin, the glutamate dehydrogenase and the triose-phosphate isomerase genes. Based on the rRNA nPCR, the presence of was confirmed in 12 (6.56%) of fecal samples, of these, five, four and three samples were positive for the , and genes, respectively. Assemblage identification by sequencing was successful in 6 of 12 samples and sequence analysis showed 100% genetic similarity with assemblage E. This observation represents the first detection of assemblage E in buffaloes calves in Brazil.
我们对巴西圣保罗州西南部地区水牛犊进行了分子特征分析。共收集了183份6月龄以下穆拉品种水牛的粪便样本。我们通过针对小亚基核糖体RNA基因的聚合酶链反应（PCR）对这些样本进行检测，并使用针对β-贾第虫、谷氨酸脱氢酶和磷酸丙糖异构酶基因部分区域的额外PCR检测对阳性样本进行特征分析。基于核糖体RNA巢式PCR，在12份（6.56%）粪便样本中确认存在[具体寄生虫名称未给出]，其中，分别有5份、4份和3份样本对[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]基因呈阳性。通过测序成功鉴定出12份样本中的6份的虫株类型，序列分析显示与虫株E具有100%的遗传相似性。这一观察结果代表了巴西首次在水牛犊中检测到虫株E。
