Arsanis Biosciences, Vienna, Austria.
College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.
Antimicrob Agents Chemother. 2018 Jun 26;62(7). doi: 10.1128/AAC.00046-18. Print 2018 Jul.
Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates.
质粒编码的多粘菌素耐药性正在肠道外致病性菌株中出现，包括流行克隆序列类型 131（ST131）-H30 菌株。Mcr-1 将磷酸乙醇胺转移到脂多糖（LPS）的脂质 A 部分，赋予多粘菌素耐药性。我们研究了这种修饰是否改变了针对 ST131 LPS O25b 侧链的单克隆抗体 ASN-4 的活性。我们证实，与多粘菌素不同，ASN-4 保留了其杀菌和内毒素中和活性，因此为对抗极其耐药的 ST131 分离株提供了一种治疗选择。
