Qin Guangqi, Su Yang, Sjöberg Trygve, Steen Stig
Department of Cardiothoracic Surgery, Lund University and Skåne University Hospital, Lund, Sweden.
Ann Transplant. 2018 Apr 24;23:268-273. doi: 10.12659/AOT.907753.
BACKGROUND The aim of this study was to investigate the oxygen consumption of explanted aerobically-perfused cardioplegic porcine hearts at different temperatures. MATERIAL AND METHODS Explanted hearts from 30 pigs weighing 50 kg were randomized into 5 groups. The hearts received continuous antegrade perfusion within a temperature-controlled sealed system. The perfusate consisted of an albumin-containing hyperoncotic cardioplegic nutrition solution with erythrocytes to a hematocrit of 10%. Five temperatures were studied: 37, 30, 22, 15, and 8°C. When the erythrocytes in the perfusate were fully saturated, the oxygenator was excluded from the circuit and blood gases were analyzed periodically until the erythrocytes had desaturated to less than 20%. Between 80% and 60% saturation the desaturation curves were linear in all groups and the oxygen consumption was calculated from this part of the curves. RESULTS The weight of the hearts was 208±4 g (mean ±SEM, n=30). The oxygen consumption in mL/min/100 g heart tissue was (mean ±SEM, n=6 in each group) 37°C: 1.10±0.04; 30°C: 0.58±0.02; 22°C: 0.33±0.01; 15°C: 0.21±0.01; and 8°C: 0.16±0.02. CONCLUSIONS The oxygen consumption of the cardioplegic perfused pig heart at normothermia was 1.1 mL/min/100 g and was reduced by 85% to 0.16 mL/min/100 g at 8°C.
背景 本研究的目的是调查在不同温度下，经体外有氧灌注的猪心脏停搏液的耗氧量。材料与方法 从30头体重50千克的猪身上取出的心脏被随机分为5组。心脏在一个温度可控的密封系统内接受持续顺行灌注。灌注液由含白蛋白的高渗心脏停搏液营养溶液和红细胞组成，血细胞比容为10%。研究了五个温度：37、30、22、15和8°C。当灌注液中的红细胞完全饱和时，将氧合器从循环中移除，并定期分析血气，直到红细胞饱和度降至20%以下。在80%至60%饱和度之间，所有组的去饱和曲线均为线性，耗氧量由此部分曲线计算得出。结果 心脏重量为208±4克（平均值±标准误，n = 30）。每100克心脏组织的耗氧量（每组n = 6，平均值±标准误）为：37°C：1.10±0.04；30°C：0.58±0.02；22°C：0.33±0.01；15°C：0.21±0.01；8°C：0.16±0.02。结论 心脏停搏液灌注的猪心脏在常温下的耗氧量为1.1毫升/分钟/100克，在8°C时降至0.16毫升/分钟/100克，降低了85%。
