Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia.
J Korean Med Sci. 2018 Apr 12;33(17):e131. doi: 10.3346/jkms.2018.33.e131. eCollection 2018 Apr 23.
Leptospirosis is a febrile zoonotic disease. Routine diagnosis of leptospirosis is based on the detection of specific antibodies with serological tests. The aim of our study was to determine the usefulness of immunochromatographic assay (ICA), ImmuneMed Leptospira IgM Duo Rapid test kit from Korea, in rapid screening of acute leptospirosis in emergency cases with limited expertise. A total of 197 serum samples (93 positive, 104 negative) were selected randomly. The test has good diagnostic sensitivity 73% and specificity 90%. With positive predictive value of 87% and negative predictive value of 79%, this reassures patients have higher chance of correct diagnosis. This ICA is acceptable for screening of leptospirosis but confirmation with microscopic agglutination test should follow.
钩端螺旋体病是一种发热性人畜共患疾病。钩端螺旋体病的常规诊断基于血清学检测来检测特定的抗体。我们的研究旨在确定免疫层析法（ICA），来自韩国的 ImmuneMed 钩端螺旋体 IgM Duo 快速检测试剂盒在具有有限专业知识的急诊情况下快速筛查急性钩端螺旋体病的有用性。总共随机选择了 197 份血清样本（93 份阳性，104 份阴性）。该检测具有良好的诊断敏感性 73%和特异性 90%。阳性预测值为 87%，阴性预测值为 79%，这使患者有更高的正确诊断机会。这种 ICA 可用于筛查钩端螺旋体病，但应随后进行显微镜凝集试验确认。
