Alejandra Sánchez-Muñoz María, Valdez-Solana Mónica Andrea, Campos-Almazán Mara Ibeth, Flores-Herrera Óscar, Esparza-Perusquía Mercedes, Olvera-Sánchez Sofia, García-Arenas Guadalupe, Avitia-Domínguez Claudia, Téllez-Valencia Alfredo, Sierra-Campos Erick
Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango Campus, Gómez Palacio, DGO, Mexico.
Facultad de Medicina y Nutrición, Universidad Juárez del Estado de Durango Campus, Durango, DGO, Mexico.
Biochem Res Int. 2018 Mar 1;2018:5681081. doi: 10.1155/2018/5681081. eCollection 2018.
The increasing prevalence of diabetes continues to be a major health issue worldwide. Alteration of mitochondrial electron transport chain is a recognized hallmark of the diabetic-associated decline in liver bioenergetics; however, the molecular events involved are only poorly understood. is used for the treatment of diabetes. However, its role on mitochondrial functionality is not yet established. This study was aimed to evaluate the effect of extract on supercomplex formation, ATPase activity, ROS production, GSH levels, lipid peroxidation, and protein carbonylation. The levels of lipid peroxidation and protein carbonylation were increased in diabetic group. However, the levels were decreased in -treated diabetic rats. Analysis of in-gel activity showed an increase in all complex activities in the diabetic group, but spectrophotometric determinations of complex II and IV activities were unaffected in this treatment. However, we found an oxygen consumption abolition through complex I-III-IV pathway in the diabetic group treated with . While respiration with succinate feeding into complex II-III-IV was increased in the diabetic group. These findings suggest that hyperglycemia modifies oxygen consumption, supercomplexes formation, and increases ROS levels in mitochondria from the liver of STZ-diabetic rats, whereas may have a protective role against some alterations.
糖尿病患病率的不断上升仍是全球主要的健康问题。线粒体电子传递链的改变是糖尿病相关肝脏生物能量学下降的一个公认标志；然而，其中涉及的分子事件仍知之甚少。[药物名称]用于治疗糖尿病。然而，其对线粒体功能的作用尚未明确。本研究旨在评估[药物名称]提取物对超复合物形成、ATP酶活性、活性氧生成、谷胱甘肽水平、脂质过氧化和蛋白质羰基化的影响。糖尿病组脂质过氧化和蛋白质羰基化水平升高。然而，在接受[药物名称]治疗的糖尿病大鼠中这些水平降低。凝胶内活性分析显示糖尿病组所有复合物活性增加，但分光光度法测定的复合物II和IV活性在该治疗中未受影响。然而，我们发现用[药物名称]治疗的糖尿病组通过复合物I - III - IV途径的氧消耗被消除。而糖尿病组中向复合物II - III - IV输入琥珀酸时的呼吸增加。这些发现表明高血糖会改变氧消耗、超复合物形成，并增加链脲佐菌素诱导的糖尿病大鼠肝脏线粒体中的活性氧水平，而[药物名称]可能对某些改变具有保护作用。
