College of Medicine, Dankook University, Beckman Laser Institute Korea, Cheonan, Republic of Korea.
Department of Otolaryngology-Head & Neck Surgery, College of Medicine, Dankook University, Cheonan, Republic of Korea.
Biomed Res Int. 2018 Mar 1;2018:9158187. doi: 10.1155/2018/9158187. eCollection 2018.
Hair cells in the cochlea can be damaged by various causes. Damaged hair cells can lead to additional destruction of parts of the auditory afferent pathway sequentially, which is called secondary degeneration. Recently, researches regarding cochlear implants have been actively carried out for clinical purposes; secondary degeneration in animals is a much more practical model for identifying the prognosis of cochlear implants. However, an appropriate model for this research is not established yet. Thus, we developed a secondary degeneration model using an ototoxic drug. 35 gerbils were separated into four different groups and kanamycin was applied via various approaches. ABR was measured several times after drug administration. SGCs were also counted to identify any secondary degeneration. The results showed that outer and inner HCs were damaged in all kanamycin-treated groups. Twelve weeks after kanamycin treatment, the round window membrane injection group showed severe subject differences in hair cells and SGC damage, whereas the gelfoam group showed consistent and severe damage in hair cells and SGCs. In this study, we successfully induced secondary degeneration in hair cells in a gerbil model. This model can be used for various purposes in the hearing research area either for treatment or for preservation.
耳蜗中的毛细胞可能会因各种原因受损。受损的毛细胞会导致听觉传入通路的其他部分依次发生进一步的破坏，这被称为继发性变性。最近，出于临床目的，人们对耳蜗植入物的研究进行了积极的研究；对于动物来说，继发性变性是一种更实用的模型，可以确定耳蜗植入物的预后。然而，该研究尚未建立合适的模型。因此，我们使用耳毒性药物开发了一种继发性变性模型。35 只沙鼠被分为四组，通过不同的途径给予卡那霉素。给药后多次测量 ABR。还计算了 SGC 以确定是否存在继发性变性。结果表明，所有卡那霉素处理组的外毛细胞和内毛细胞均受损。卡那霉素治疗 12 周后，圆窗膜注射组的毛细胞和 SGC 损伤在个体间差异显著，而明胶海绵组的毛细胞和 SGC 损伤一致且严重。在这项研究中，我们成功地在沙鼠模型中诱导了毛细胞的继发性变性。该模型可用于听力研究领域的各种目的，无论是治疗还是保存。
