Bascom Palmer Eye Institute, University of Miami, Miller School of Medicine, Miami, FL 33136.
Department of Ophthalmology, First Affiliated Hospital, Dalian Medical University, Dalian, 116011, China.
eNeuro. 2018 Apr 20;5(2). doi: 10.1523/ENEURO.0109-18.2018. eCollection 2018 Mar-Apr.
Retinal degenerations are a major cause of vision impairment and blindness. Neuroprotective therapy is a promising therapeutic strategy for retinal degenerative diseases. We investigated a novel neurotrophic factor mesencephalic astrocyte-derived neurotrophic factor (MANF) in the retina. MANF is expressed at a high level during postnatal development and the expression declines to a lower level as the retina matures. Müller cells are the major cells expressing MANF. It is also found in the retinal ganglion cells, in the inner nuclear layer (INL) neurons, and in retinal pigment epithelial (RPE) cells. Intravitreal injection of recombinant human (rh)MANF significantly protected rod and cone photoreceptors in rats carrying the rhodopsin S334ter mutation, and preserved electroretinograms (ERGs) in the ( ) mice. These results indicate that MANF is a native protein in the retina and is a potent neurotrophic factor for photoreceptor protection.
视网膜变性是视力损害和失明的主要原因。神经保护疗法是治疗视网膜变性疾病的一种很有前途的治疗策略。我们研究了一种新型神经营养因子——中脑神经胶质细胞衍生神经营养因子 (MANF) 在视网膜中的作用。MANF 在出生后发育过程中表达水平较高，随着视网膜的成熟，其表达水平下降到较低水平。Müller 细胞是表达 MANF 的主要细胞。它也存在于视网膜神经节细胞、内核层 (INL) 神经元和视网膜色素上皮 (RPE) 细胞中。玻璃体内注射重组人 (rh)MANF 可显著保护携带 rhodopsin S334ter 突变的大鼠的 rod 和 cone 光感受器，并保持 ( ) 小鼠的视网膜电图 (ERGs)。这些结果表明，MANF 是视网膜中的一种天然蛋白，是光感受器保护的有效神经营养因子。
