使用从二氧化锡锗/镓发生器中获得的经阳离子交换树脂纯化的镓-68洗脱液优化 Ga-68 标记 DOTATATE 的标记和试剂盒制备方法。
Optimization of a Labeling and Kit Preparation Method for Ga-68 Labeled DOTATATE, Using Cation Exchange Resin Purified Ga-68 Eluates Obtained from a Tin Dioxide Ge/Ga Generator.
机构信息
Radionuclide Production Department, iThemba LABS, PO Box 722, Somerset West, 7129, South Africa.
Nuclear Medicine Division, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, 8000, South Africa.
出版信息
Mol Imaging Biol. 2018 Dec;20(6):1008-1014. doi: 10.1007/s11307-018-1195-x.
PURPOSE
The aim of this study was to optimize a radiolabeling method using cationic processed Ga-68 eluates from a SnO-based Ge/Ga generator, followed by the development of DOTA-Tyr-Thre-octreotide (DOTATATE) kits.
PROCEDURES
Diluted generator eluates were adsorbed on a SCX resin and desorbed with acidified 5 M NaCl solution. Optimized labeling conditions were determined by variation of pH, using 35 μg DOTATATE and sodium acetate buffer. DOTATATE kits were developed based on optimized radiolabeling conditions, were labeled, and evaluated.
RESULTS
Optimized labeling conditions resulted in a radiolabeling efficiency of around 99 % and radiochemical yield of almost 85 %. Different kit preparation methods did not significantly influence the radiolabeling results. Kits were found to be stable over 3 months.
CONCLUSION
A labeling method using SCX-processed Ga-68 eluates was optimized. DOTATATE kits specifically for these SCX-processed Ga-68 eluates were successfully formulated. A post-labeling Sep-Pak C18 purification should be optional.
目的
本研究旨在优化一种使用基于 SnO 的 Ge/Ga 发生器的阳离子处理 Ga-68 洗脱液进行放射性标记的方法，随后开发 DOTA-Tyr-Thre-octreotide（DOTATATE）试剂盒。
过程
稀释的发生器洗脱液被吸附在 SCX 树脂上，并用酸化的 5 M NaCl 溶液洗脱。通过改变 pH 值来确定优化的标记条件，使用 35μg DOTATATE 和乙酸钠缓冲液。基于优化的放射性标记条件开发了 DOTATATE 试剂盒，对其进行了标记和评估。
结果
优化的标记条件得到了约 99%的放射性标记效率和约 85%的放射化学收率。不同的试剂盒制备方法对标记结果没有显著影响。试剂盒在 3 个月以上的时间内保持稳定。
结论
优化了一种使用 SCX 处理的 Ga-68 洗脱液的标记方法。成功地为这些 SCX 处理的 Ga-68 洗脱液专门配制了 DOTATATE 试剂盒。可以选择在标记后进行 Sep-Pak C18 纯化。
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