Cheng Wenjie, Pan Jiahong, Yang Jianying, Zheng Zengyao, Lu Fushen, Chen Yaowen, Gao Wenhua
Department of Chemistry and Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong, 515063, People's Republic of China.
National Detergents and Cosmetics Products Quality Supervision and Inspection Center (Guangdong), Shantou, Guangdong, 515041, People's Republic of China.
Mikrochim Acta. 2018 Apr 23;185(5):263. doi: 10.1007/s00604-018-2800-z.
A photoelectrochemical (PEC) aptasensor for the highly sensitive and specific detection of thrombin is described. This aptasensor is based on an indium tin oxide (ITO) support that is covered with carbon quantum dot (CQD)-sensitized TiO and acts as a photoactive matrix. The ITO/TiO/CQD electrode was prepared by impregnation assembly. It displays an enhanced and steady photocurrent response under irradiation by visible light. A carboxyl-functionalized thrombin-binding aptamer was covalently immobilized on the modified ITO to obtain a PEC aptasensor whose photocurrent decreases with increasing concentration of thrombin. Under 420 nm irradiation at a bias voltage of 0 V, the aptasensor has a linear response in the 1.0 to 250 pM thrombin concentration range, with a 0.83 pM detection limit. Conceivably, this approach can be extended to numerous other PEC aptasensors for the detection of targets for which appropriate aptamers are available. Graphical abstract Schematic of a PEC aptasensor for thrombin. It is based on the use of CQD as the sensitizer, TiO/CQDs as the photoactive matrix, and the thrombin aptamer as the recognition element.
描述了一种用于高灵敏度和特异性检测凝血酶的光电化学（PEC）适配体传感器。该适配体传感器基于覆盖有碳量子点（CQD）敏化的TiO的氧化铟锡（ITO）载体，其作为光活性基质。ITO/TiO/CQD电极通过浸渍组装制备。在可见光照射下，它显示出增强且稳定的光电流响应。将羧基功能化的凝血酶结合适配体共价固定在修饰的ITO上，以获得一种PEC适配体传感器，其光电流随凝血酶浓度的增加而降低。在0 V偏置电压下420 nm光照下，该适配体传感器在1.0至250 pM凝血酶浓度范围内具有线性响应，检测限为0.83 pM。可以想象，这种方法可以扩展到许多其他PEC适配体传感器，用于检测有合适适配体可用的靶标。图形摘要：凝血酶PEC适配体传感器的示意图。它基于使用CQD作为敏化剂，TiO/CQDs作为光活性基质，以及凝血酶适配体作为识别元件。
