Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, State Key Laboratory Base of Eco-Chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Mikrochim Acta. 2024 Mar 22;191(4):216. doi: 10.1007/s00604-024-06279-3.
A photoelectrochemical (PEC) sensor for the sensitive detection of thrombin (TB) was established. Co-sensitized combination of TiO nanoparticles combined with modified cadmium sulfide and cadmium telluride quantum dots (CdS/CdTe QDs) was utilized as a photoactive material. Successful growth of CdS/CdTe quantum dots on mesoporous TiO films occured by successive ion-layer adsorption and reaction. This interesting formation of co-sensitive structure is conducive to enhancing the photocurrent response by improving the use rate of light energy. Additionally, the step-level structure of CdS/CdTe QDs and TiO NPs shows a wide range of visible light absorption, facilitating the dissociation of excitons into free electrons and holes. Consequently, the photoelectric response of the PEC analysis platform is significantly enhanced. This constructed PEC aptasensor shows good detection of thrombin with a low detection limit (0.033 pM) and a wide linear range (0.0001-100 nM) in diluted actual human serum samples. In addition, this PEC aptasensor also has the characteristics of good stability and good reproducibility, which provides a novel insight for the quantitative measurement of other similar analytes.
建立了一种用于灵敏检测凝血酶（TB）的光电化学（PEC）传感器。将 TiO 纳米粒子与修饰的硫化镉和碲化镉量子点（CdS/CdTe QDs）协同敏化组合用作光活性材料。通过连续离子层吸附和反应成功地在介孔 TiO 薄膜上生长了 CdS/CdTe 量子点。这种共敏化结构的有趣形成有利于通过提高光能利用率来增强光电流响应。此外，CdS/CdTe QDs 和 TiO NPs 的阶跃结构表现出宽范围的可见光吸收，有利于激子离解成自由电子和空穴。因此，PEC 分析平台的光电响应得到显著增强。所构建的 PEC 适体传感器在稀释的实际人血清样品中对凝血酶具有良好的检测性能，检测限低（0.033 pM），线性范围宽（0.0001-100 nM）。此外，这种 PEC 适体传感器还具有良好的稳定性和重现性的特点，为定量测量其他类似分析物提供了新的思路。
