López-Zapata Antonio, León-Navarro David Agustín, Crespo María, Martín Mairena
Departamento de Química Inorgánica, Orgánica y Bioquímica, Facultad de Ciencias y Tecnologías Químicas/Facultad de Medicina de Ciudad Real, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Avenida Camilo José Cela, 10, 13071 Ciudad Real, Spain.
Departamento de Química Inorgánica, Orgánica y Bioquímica, Facultad de Ciencias y Tecnologías Químicas/Facultad de Medicina de Ciudad Real, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Avenida Camilo José Cela, 10, 13071 Ciudad Real, Spain.
Int J Dev Neurosci. 2018 Aug;68:10-16. doi: 10.1016/j.ijdevneu.2018.04.004. Epub 2018 Apr 22.
In the present work we have studied the effect of maternal intake of l-Glutamate (l-Glu) (1 g/L) during lactation on group I mGluR transduction pathway in brain plasma membrane from 15 days-old neonates. Results obtained have shown that maternal l-glutamate intake did not significantly affect neither weights of pups nor negative geotaxis reflex, an index of neurobehavioral development, but increased l-Glu plasma level in both male and female neonates. In male neonates, maternal l-Glu intake evoked a loss of mGluR whereas no variation on mGluR was observed as revealed by Western-blotting assay. The loss of mGluR was accompanied by a decrease on l-Glu-stimulated phospholipase C activity suggesting, therefore, a loss of group I mGluR functionality. Concerning female neonates, no variations were detected neither mGluR nor mGluR and group I mGluR functionality was also preserved.
在本研究中，我们探究了哺乳期母体摄入L-谷氨酸（L-Glu，1 g/L）对15日龄新生儿脑细胞膜中I组代谢型谷氨酸受体（mGluR）转导途径的影响。结果表明，母体摄入L-谷氨酸对幼崽体重及负趋地性反射（神经行为发育指标）均无显著影响，但会使雄性和雌性新生儿的血浆L-谷氨酸水平升高。在雄性新生儿中，母体摄入L-谷氨酸导致mGluR缺失，而蛋白质免疫印迹分析显示mGluR无变化。mGluR的缺失伴随着L-谷氨酸刺激的磷脂酶C活性降低，因此表明I组mGluR功能丧失。对于雌性新生儿，未检测到mGluR有变化，I组mGluR功能也得以保留。
