Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padua, Italy.
Food Chem Toxicol. 2018 Jun;116(Pt B):335-345. doi: 10.1016/j.fct.2018.04.047. Epub 2018 Apr 22.
In the present study, the antioxidant activity, anti-glycation activity, α-amylase, α-glucosidase inhibitory activity of polysaccharides from Inonotus obliquus (UIOPS) and its chromium (III) complex (UIOPC) were investigated. Their protective effects against HO-induced oxidative damages in hepatic L02 cells were also assessed. Results demonstrated that UIOPC and UIOPS exhibited remarkable DPPH scavenging activity, ferric reducing power and hemolysis inhibitory activity. UIOPC also showed significant inhibitory capacity on α-amylase and α-glucosidase than UIOPS (P < 0.05), suggesting a good regulation of the postprandial hyperglycemia. Three phases of advanced glycation end products (AGEs) formation were effectively inhibited by UIOPC and UIOPS. Moreover, pretreatment with UIOPC and UIOPS markedly attenuated the oxidative damage induced by HO in hepatic L02 cells via enhancing the cell viability, inhibiting the morphology alteration and maintaining the integrity of mitochondria. These results indicated that the anti-diabetic mechanism of UIOPC might involve in the homoeostasis of blood glucose and the recovery of endogenous antioxidant system. The elucidation of the potential anti-diabetic mechanism will facilitate the further study and application of the polysaccharides-metal complex in the functional food industry.
本研究旨在探讨桦褐孔菌多糖（UIOP）及其三价铬（III）络合物（UIOPC）的抗氧化活性、抗糖基化活性、α-淀粉酶、α-葡萄糖苷酶抑制活性，并评估其对 HO 诱导的肝 L02 细胞氧化损伤的保护作用。结果表明，UIOPC 和 UIOPS 具有显著的 DPPH 清除活性、铁还原能力和溶血抑制活性。与 UIOPS 相比，UIOPC 对α-淀粉酶和α-葡萄糖苷酶也表现出显著的抑制能力（P<0.05），提示其对餐后高血糖有良好的调节作用。UIOPC 和 UIOPS 能有效抑制晚期糖基化终产物（AGEs）形成的三个阶段。此外，UIOPC 和 UIOPS 预处理可通过提高细胞活力、抑制形态改变和维持线粒体完整性，显著减轻 HO 诱导的肝 L02 细胞氧化损伤。这些结果表明，UIOPC 的抗糖尿病机制可能涉及血糖稳态和内源性抗氧化系统的恢复。阐明其潜在的抗糖尿病机制将有助于进一步研究和应用该多糖-金属络合物于功能性食品工业。
