Wang Cong, Chen Zhongqin, Pan Yuxiang, Gao Xudong, Chen Haixia
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
Food Chem Toxicol. 2017 Oct;108(Pt B):498-509. doi: 10.1016/j.fct.2017.01.007. Epub 2017 Jan 11.
Polysaccharides are important bioactive ingredients from Inonotus obliquus. This study aimed to synthesize and characterize a novel I. obliquus polysaccharides-chromium (III) complex (UIOPC) and investigate the anti-diabetic effects in streptozotocin (STZ) induced type 2 diabetes mellitus (T2DM) mice and sub-acute toxicity in normal mice. The molecular weight of UIOPC was about 11.5 × 10 Da with the chromium content was 13.01% and the chromium was linked with polysaccharides through coordination bond. After treatment of UIOPC for four weeks, the body weight, fasting blood glucose (FBG) levels, plasma insulin levels of the diabetic mice were significantly reduced when compared with those of the diabetic mice (p < 0.05). The results on serum profiles and antioxidant enzymes activities revealed that UIOPC had a positive effect on hypoglycemic and antioxidant ability. Histopathology results showed that UIOPC could effectively alleviate the STZ-lesioned tissues in diabetic mice. Furthermore, high dose administration of UIOPC had no obviously influence on serum profiles levels and antioxidant ability of the normal mice and the organ tissues maintained organized and integrity in the sub-acute toxicity study. These results suggested that UIOPC might be a good candidate for the functional food or pharmaceuticals in the treatment of T2DM.
多糖是桦褐孔菌中的重要生物活性成分。本研究旨在合成并表征一种新型桦褐孔菌多糖 - 铬（III）配合物（UIOPC），并研究其对链脲佐菌素（STZ）诱导的2型糖尿病（T2DM）小鼠的抗糖尿病作用以及对正常小鼠的亚急性毒性。UIOPC的分子量约为11.5×10 Da，铬含量为13.01%，铬通过配位键与多糖相连。用UIOPC治疗四周后，与糖尿病小鼠相比，糖尿病小鼠的体重、空腹血糖（FBG）水平、血浆胰岛素水平显著降低（p <0.05）。血清学指标和抗氧化酶活性结果表明，UIOPC对降血糖和抗氧化能力有积极作用。组织病理学结果显示，UIOPC可有效减轻糖尿病小鼠的STZ损伤组织。此外，在亚急性毒性研究中，高剂量给予UIOPC对正常小鼠的血清学指标水平和抗氧化能力没有明显影响，器官组织保持有组织和完整。这些结果表明，UIOPC可能是治疗T2DM的功能性食品或药物的良好候选者。
