Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, PR China.
Sichuan Center for Disease Control and Prevention, Chengdu, 610041, PR China.
Colloids Surf B Biointerfaces. 2018 Jul 1;167:354-363. doi: 10.1016/j.colsurfb.2018.04.029. Epub 2018 Apr 16.
This work focuses on the optimization design of a functional biomimetic scaffold for the repair of osteochondral defects and includes the study of single integrated osteochondral tissue engineering scaffolds with a multi-layered functional structure. Rabbit model experiments were used to evaluate the repair of osteochondral defects. The results revealed that good integration was achieved both at the interfaces between the scaffold material and the host tissue and between the newly formed subchondral bone and cartilage. The highest total histological score of 24.2 (based on the modified O'Driscoll scoring system at 12 weeks post-operation) was achieved for osteochondral repair. The completely repaired cylindrical full-thickness defects for the rabbit animal model reached 5 mm in diameter. The thickness of the regenerated cartilage was almost in line with that of the surrounding normal cartilage, the number and arrangement of cells in the superficial area of cartilage were very close to those of normal hyaline cartilage, and there were clear cartilage lacunas in the regenerated cartilage. The hybrid-use of growth factors and LIPUS stimulation exhibited good potential in enhancing vascularization and the formation of new bone and cartilage, providing better conditions for the overall osteochondral repair.
本工作致力于优化设计一种用于修复骨软骨缺损的功能性仿生支架，包括研究具有多层功能结构的单一集成式骨软骨组织工程支架。采用兔模型实验对骨软骨缺损的修复进行了评估。结果表明，支架材料与宿主组织之间以及新形成的软骨下骨与软骨之间均实现了良好的整合。术后 12 周，采用改良 O'Driscoll 评分系统，骨软骨修复的总组织学评分为 24.2 分（最高）。对于兔动物模型的完全修复的圆柱形全层厚度缺损，直径达到 5mm。再生软骨的厚度几乎与周围正常软骨一致，软骨浅层的细胞数量和排列与正常透明软骨非常接近，并且在再生软骨中存在清晰的软骨陷窝。生长因子与 LIPUS 刺激的混合使用在增强血管生成和新骨与软骨形成方面表现出良好的潜力，为整体骨软骨修复提供了更好的条件。
