Maghazachi A A, Phillips-Quagliata J M
Department of Pathology, New York University Medical Center.
Int Arch Allergy Appl Immunol. 1988;86(2):147-56. doi: 10.1159/000234564.
Four BALB/c T cell clones from among a set propagated in the presence of concanavalin A (Con A) were selected on the basis of their ability to produce supernatant factors promoting high IgA plaque-forming cell (PFC) responses by 2,4,6-trinitrophenyl-conjugated keyhole limpet hemocyanin (TNP-KLH)-primed splenic B cells in the presence of TNP-SRBC. Such clones could be derived from cultures containing T cells not only from gut-associated lymphoid tissue, but also from the spleen. The selected clones all proliferated well in the presence of syngeneic, irradiated APC without either Con A or exogenous IL-2, but required both APC and Con A to produce helper factors. Factors from three of the clones helped B cells both to proliferate and to differentiate into IgM, IgG and IgA PFC. Factors from the fourth clone helped B cells differentiate into IgA and IgG PFC and may have promoted switching to these isotypes but did not support either B cell proliferation or generation of IgM PFC. Cross-linking of B cell receptors for antigen was not required for the response to the helper factors since TNP-SRBC were unnecessary and high concentrations of them were actually inhibitory.
从在伴刀豆球蛋白A（Con A）存在下传代培养的一组细胞中挑选出4个BALB/c T细胞克隆，挑选依据是它们能够产生上清因子，在三硝基苯偶联的钥孔戚血蓝蛋白（TNP-KLH）致敏的脾B细胞存在且有TNP-绵羊红细胞（TNP-SRBC）的情况下，促进高IgA空斑形成细胞（PFC）反应。这样的克隆不仅可来源于含有来自肠道相关淋巴组织的T细胞的培养物，也可来源于脾脏T细胞的培养物。所选克隆在同基因、经辐照的抗原呈递细胞（APC）存在下，无需Con A或外源性白细胞介素-2（IL-2）即可良好增殖，但产生辅助因子需要APC和Con A两者。来自三个克隆的因子可帮助B细胞增殖并分化为IgM、IgG和IgA PFC。来自第四个克隆的因子可帮助B细胞分化为IgA和IgG PFC，可能促进向这些同种型的转换，但不支持B细胞增殖或IgM PFC的产生。对辅助因子的反应不需要抗原的B细胞受体交联，因为TNP-SRBC并非必需，而且高浓度的TNP-SRBC实际上具有抑制作用。
