Suzuki I, Kitamura K, Kiyono H, Kurita T, Green D R, McGhee J R
J Exp Med. 1986 Aug 1;164(2):501-16. doi: 10.1084/jem.164.2.501.
The ability of murine Peyer's patch (PP) T contrasuppressor cells (Tcs) to reverse oral tolerance to the T cell-dependent (TD) antigen SRBC was studied both in vivo and in vitro. C3H/HeJ mice given SRBC orally for 4 wk are not rendered tolerant to this antigen and were used as a source of PP Tcs cells for adoptive transfer to identically treated, orally tolerized C3H/HeN mice. Transfer of 10(4) or 5 X 10(4) V. villosa-adherent PP T cells resulted in splenic IgM, IgG, and mainly IgA responses in C3H/HeN mice challenged systemically with SRBC. The T cell responsible was Lyt-1+, 2-, L3T4-, I-JK+ and V. villosa lectin-adherent, all characteristics of mature effector Tcs cells. This C3H/HeJ PP Tcs cell subset was also effective when added to in vitro cultures of tolerized spleen cells derived from SRBC-fed, C3H/HeN mice. Interestingly, C3H/HeJ PP Tcs cells restored mainly IgA responses when transferred in vivo or when added to suppressed C3H/HeN splenic cultures. Comparison of the functional activity of Tcs cells derived from spleen or PP of orally immunized C3H/HeJ mice revealed that splenic Tcs cells supported responses of all 3 isotypes; however, PP Tcs cells yielded three-fourfold higher IgA responses, when compared with IgM or IgG anti-SRBC responses. Adherence of C3H/HeJ PP Tcs to an Fc alpha R+ T cell line derived from IgA-specific Th cells resulted in a nonadherent cell fraction that potentiated only IgM and IgG responses, while bound Tcs cells preferentially supported IgA responses. These results suggest that murine PP contain IgA-specific Tcs cells that allow IgA response induction in the presence of Ts cells that mediate oral tolerance.
对小鼠派伊尔结（PP）T 辅助抑制细胞（Tcs）逆转对 T 细胞依赖性（TD）抗原绵羊红细胞（SRBC）口服耐受的能力进行了体内和体外研究。口服 SRBC 4 周的 C3H/HeJ 小鼠对该抗原未产生耐受，这些小鼠被用作 PP Tcs 细胞的来源，用于过继转移到经相同处理且口服耐受的 C3H/HeN 小鼠体内。转移 10⁴ 或 5×10⁴ 个绒毛状黏附的 PP T 细胞，可使经 SRBC 全身攻击的 C3H/HeN 小鼠产生脾脏 IgM、IgG，主要是 IgA 应答。起作用的 T 细胞为 Lyt-1⁺、2⁻、L3T4⁻、I-JK⁺ 且为绒毛状凝集素黏附型，这些都是成熟效应 Tcs 细胞的特征。当将该 C3H/HeJ PP Tcs 细胞亚群添加到来自喂食 SRBC 的 C3H/HeN 小鼠的耐受脾细胞的体外培养物中时，也具有效果。有趣的是，C3H/HeJ PP Tcs 细胞在体内转移或添加到受抑制 的C3H/HeN 脾脏培养物中时，主要恢复 IgA 应答。对口服免疫的 C3H/HeJ 小鼠脾脏或 PP 来源的 Tcs 细胞的功能活性进行比较发现，脾脏 Tcs 细胞支持所有三种同种型的应答；然而，与 IgM 或 IgG 抗 SRBC 应答相比，PP Tcs 细胞产生的 IgA 应答高出三倍。C3H/HeJ PP Tcs 与源自 IgA 特异性 Th 细胞的 FcαR⁺ T 细胞系黏附后，产生的非黏附细胞部分仅增强 IgM 和 IgG 应答，而结合的 Tcs 细胞则优先支持 IgA 应答。这些结果表明，小鼠 PP 中含有 IgA 特异性 Tcs 细胞，在介导口服耐受的 Ts 细胞存在的情况下，这些细胞可诱导 IgA 应答。
