Bianchi R, Romani L, Puccetti P, Fioretti M C
Institute of Pharmacology, University of Perugia, Italy.
Int J Cancer. 1988 Jul 15;42(1):71-5. doi: 10.1002/ijc.2910420114.
Splenocytes immune to a highly immunogenic ("xenogenized", L5178Y/DTIC) variant of a murine lymphoma exert anti-parental-tumor activity in a systemic adoptive transfer system, the effect being apparently associated with the Lyt-2- fraction of the lymphocyte population. During investigation of the mechanisms of this protection, we found that the L5178Y/DTIC tumor-immune lymphocytes exhibited an appreciable anti-L5178Y delayed-type hypersensitivity (DTH) response. Enrichment of those lymphocytes in L3T4+ cells significantly enhanced the protective effect as well as the DTH reaction, whereas the use of an anti-L3T4 but not anti-Lyt-2 reagent blocked both activities. In vitro, lymphocyte proliferation against L5178Y cells occurred and was apparently associated with the Lyt-2- fraction of a population of L5178Y/DTIC immune splenocytes.
对鼠淋巴瘤的一种高免疫原性（“异种源化”，L5178Y/DTIC）变体产生免疫的脾细胞，在全身过继性转移系统中发挥抗亲代肿瘤活性，该效应显然与淋巴细胞群体的Lyt-2阴性部分相关。在研究这种保护机制的过程中，我们发现L5178Y/DTIC肿瘤免疫淋巴细胞表现出明显的抗L5178Y迟发型超敏反应（DTH）。L3T4⁺细胞中这些淋巴细胞的富集显著增强了保护作用以及DTH反应，而使用抗L3T4试剂而非抗Lyt-2试剂会阻断这两种活性。在体外，针对L5178Y细胞的淋巴细胞增殖发生，且显然与L5178Y/DTIC免疫脾细胞群体的Lyt-2阴性部分相关。
