Vidigal Izadora, De Carvalho Thiago R, Clemente-Carvalho Rute B G, Giaretta Ariovaldo A
Laboratório de Taxonomia, Sistemática e Evolução de Anuros Neotropicais, Universidade Federal de Uberlândia (UFU), campus Ituiutaba, Ituiutaba, MG, Brazil. Programa de Pós-Graduação em Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (Unicamp), Campinas, SP, Brazil..
Zootaxa. 2018 Feb 28;4388(1):61-75. doi: 10.11646/zootaxa.4388.1.4.
Crossodactylus werneri was described based on specimens collected in the 1970's at Parque Nacional do Itatiaia, being also reported for nearby localities. We collected specimens that we assigned to C. werneri, and recorded calls of the species during fieldworks at Serra das Cabras (Campinas, state of São Paulo). In this paper, we describe for the first time the vocalizations, tadpole, coloration in life, and comment on aspects of the natural history of C. werneri. Besides, the examination of specimens in zoological collections allowed us to extend the geographic range for this species. We also make remarks on morphological/chromatic variation and provide 16S rDNA sequences for the species. Adults were found along a slow-flowing streamlet with sandy/muddy bottom within a small fragment of secondary forest. Males called between sunset and first hours of the night. Advertisement call consisted of series of pulsed notes. Call duration lasted around 3 s, emitted at the highest rate of 17 calls per minute and six notes per second. Note duration lasted around 18 ms. Notes had poorly defined pulses (irregular and/or weak amplitude modulations along the note). The dominant frequency was about 3380 Hz. Territorial call had a long, well-defined pulsed portion followed by a higher-amplitude "squeak". The dominant frequency was around 3400 Hz. Tadpoles were essentially similar to those of other Crossodactylus species, except by not having nostril ornamentation. Our record of C. werneri in Serra das Cabras might be regarded a rediscovery of this species since C. werneri had not been recorded for more than 30 years until our first record of C. werneri in the field from 2011 and subsequent years. Our record is approximately 100 km west, and Mococa 200 km northwest, from Santo Antônio do Pinhal, the westernmost previous record for C. werneri up to date. Gene sequences (16S rRNA) give insights into the genetic divergence between C. werneri and some congeners.
韦氏胯蛙是根据20世纪70年代在伊塔蒂亚伊国家公园采集的标本描述的，附近地区也有相关报道。我们采集了被认定为韦氏胯蛙的标本，并在卡布拉斯山脉（圣保罗州坎皮纳斯）的野外工作中记录了该物种的叫声。在本文中，我们首次描述了韦氏胯蛙的鸣声、蝌蚪、活体颜色，并对其自然史的一些方面进行了评论。此外，对动物标本馆中的标本进行检查使我们能够扩大该物种的地理分布范围。我们还对形态/颜色变异进行了评论，并提供了该物种的16S rDNA序列。成体在一小片次生林内一条底部为沙质/泥质的缓流小溪中被发现。雄性在日落至夜间的最初几个小时鸣叫。求偶叫声由一系列脉冲音组成。叫声持续时间约为3秒，最高发出频率为每分钟17次叫声、每秒6个音。音的持续时间约为18毫秒。音的脉冲定义不清晰（沿音有不规则和/或微弱的振幅调制）。主频约为3380赫兹。领地叫声有一个长的、定义明确的脉冲部分，随后是一个振幅更高的“吱吱声”。主频约为3400赫兹。蝌蚪与其他胯蛙属物种的蝌蚪基本相似，只是没有鼻孔装饰。我们在卡布拉斯山脉对韦氏胯蛙的记录可能被视为该物种的重新发现，因为在我们2011年及随后几年在野外首次记录到韦氏胯蛙之前，已有30多年未记录到该物种。我们的记录地点在最西端此前韦氏胯蛙记录地点圣安东尼奥-杜皮尼亚尔以西约100公里处，西北偏北200公里处是莫科卡。基因序列（16S rRNA）揭示了韦氏胯蛙与一些同属物种之间的遗传差异。
