Tangkawanit Ubon, Wongpakam Komgrit, Pramual Pairot
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002 Thailand..
Zootaxa. 2018 Feb 28;4388(1):111-122. doi: 10.11646/zootaxa.4388.1.8.
A new black fly species of the subgenus Asiosimulium Takaoka Choochote of the genus Simulium was recognized from northeastern Thailand based on morphology, mitochondrial DNA and ecology. This black fly species has similar morphological characteristics to Simulium oblongum Takaoka and Choochote that was also described from the same geographic region in all life stages. However, this new species could be distinguished at the adult stage by coloration of the maxillary palp and in the larval stage by the presence of a pigmented subesophageal ganglion that is lacking in S. oblongum. Genetic distance and phylogenetic analyses based on mitochondrial cytochrome c oxidase I sequences clearly differentiated the two species with minimum genetic distance of 3.51%. These species are also ecologically isolated as S. oblongum is found only at low elevation (<650 m above sea level) but the new species occurs only at high elevation (>1,100 m above sea level).
基于形态学、线粒体DNA和生态学，在泰国东北部发现了蚋属阿氏蚋亚属的一种新的黑蝇物种。这种黑蝇物种在所有生命阶段都具有与同样来自同一地理区域的长形蚋相似的形态特征。然而，这个新物种在成虫阶段可通过上颌触须的颜色来区分，在幼虫阶段可通过存在一个色素沉着的咽下神经节来区分，而长形蚋则没有。基于线粒体细胞色素c氧化酶I序列的遗传距离和系统发育分析清楚地将这两个物种区分开来，最小遗传距离为3.51%。这两个物种在生态上也相互隔离，因为长形蚋仅在低海拔（海拔低于650米）被发现，而新物种仅在高海拔（海拔高于1100米）出现。
