Evans C H, Baker P D
Division of Cancer Etiology, National Cancer Institute, Bethesda, MD 20892.
J Natl Cancer Inst. 1988 Aug 3;80(11):861-4. doi: 10.1093/jnci/80.11.861.
The uptake of tumor-inhibitory antibiotics by human K562 erythroleukemia cells was examined in the presence of leukoregulin to determine if the lymphokine's ability to increase the plasma membrane permeability of tumor cells facilitates concurrent entry of pharmacologically active molecules. K562 cells were exposed to 0.25-2.0 micrograms of doxorubicin/mL for up to 60 minutes at 37 degrees C. Commencing within 15 minutes, leukoregulin increased the entry of doxorubicin approximately twofold and the uptake of mitomycin, mithramycin, and propidium iodide twofold to tenfold. This finding indicates the potential biotherapeutic value of leukoregulin in promoting the selective entry of pharmacologically active molecules into leukoregulin-sensitive target cells.
在白细胞调节素存在的情况下，检测人K562红白血病细胞对肿瘤抑制性抗生素的摄取情况，以确定这种淋巴因子增加肿瘤细胞质膜通透性的能力是否有助于药理活性分子的同时进入。将K562细胞在37℃下暴露于0.25 - 2.0微克/毫升的阿霉素中长达60分钟。在15分钟内，白细胞调节素使阿霉素的进入量增加了约两倍，使丝裂霉素、光神霉素和碘化丙啶的摄取量增加了两倍至十倍。这一发现表明白细胞调节素在促进药理活性分子选择性进入对白细胞调节素敏感的靶细胞方面具有潜在的生物治疗价值。
