Lu F C, Xie X Y, Yin X M, Gao Y J
Dental Center, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Department of Stomatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2018 Apr 9;53(4):254-258. doi: 10.3760/cma.j.issn.1002-0098.2018.04.008.
To examine the expression and potential clinical significance of CCT (cytidine triphosphate: phosphocholine cytidylyltransferase)-α in oral squamous cell carcinoma (OSCC). Fifty-eight OSCC and paired adjacent non-malignant epithelia samples (between May 2016 and July 2016) were obtained from dental center, Second Xiangya Hospital, Central South University. CCT-α expression was examined by immunohistochemistry. The relationship between CCT-α and clinicopathological features of OSCC patients was analyzed. Quantitative real-time PCR and Western blot were performed to measure the expression of CCT-α mRNA and protein level in several OSCC cell line and two normal oral epithelial cell line. Immunohistochemistry showed that CCT-α positive staining was found in cell nuclear of OSCC cells and adjacent epithelial cells. CCT-α was positively expressed in OSCC, which was significantly higher than that adjacent to carcinoma tissues (0.000). The expression of CCT-α in oral squamous cell carcinoma was correlated with smoking, alcohol consumption, tumor size, differentiation degree and lymph node metastasis. The expression level of CCT-α protein was significantly increased in patients with a history of smoking and alcohol consumption (0.001, 0.004). With the increase of tumor diameter, the expression of CCT-α protein was significantly increased (0.005). According to histopathological grade, the lower the degree of tumor differentiation, the higher the expression level of CCT-α protein (0.000). The expression of CCT-α protein was significantly higher in patients with lymph node metastasis compared with no lymph node metastasis (0.000). Quantitative real-time PCR results showed the CCT-α mRNA expression level was significantly higher in OSCC cells than that in normal oral epithelial cells (0.016). The protein expression level of CCT-α was significantly higher in OSCC cells than that in normal oral epithelial cells. CCT-α may play a critical role in the carcinogenesis and development of OSCC.
磷酸胆碱胞苷转移酶（CCT）-α在口腔鳞状细胞癌（OSCC）中的表达及潜在临床意义。2016年5月至2016年7月期间，从中南大学湘雅二医院牙科中心获取了58例OSCC及配对的相邻非恶性上皮组织样本。采用免疫组织化学法检测CCT-α的表达。分析CCT-α与OSCC患者临床病理特征之间的关系。进行定量实时聚合酶链反应和蛋白质免疫印迹法，以检测几种OSCC细胞系和两种正常口腔上皮细胞系中CCT-α mRNA和蛋白水平的表达。免疫组织化学显示，在OSCC细胞和相邻上皮细胞的细胞核中发现CCT-α阳性染色。CCT-α在OSCC中呈阳性表达，显著高于癌旁组织（0.000）。口腔鳞状细胞癌中CCT-α的表达与吸烟、饮酒、肿瘤大小、分化程度及淋巴结转移相关。有吸烟和饮酒史的患者中，CCT-α蛋白表达水平显著升高（0.001，0.004）。随着肿瘤直径增大，CCT-α蛋白表达显著增加（0.005）。根据组织病理学分级，肿瘤分化程度越低，CCT-α蛋白表达水平越高（0.000）。有淋巴结转移的患者中CCT-α蛋白表达显著高于无淋巴结转移者（0.000）。定量实时聚合酶链反应结果显示，OSCC细胞中CCT-α mRNA表达水平显著高于正常口腔上皮细胞（0.016）。OSCC细胞中CCT-α蛋白表达水平显著高于正常口腔上皮细胞。CCT-α可能在OSCC的发生发展中起关键作用。
