Wang Run, Cai Bohui, Xiang Juan, Zhu Xinwei, Jiang Yong
Department of Preventive Dentistry, Stomatologic Hospital and College, Anhui Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2016 Mar;51(3):137-42. doi: 10.3760/cma.j.issn.1002-0098.2016.03.003.
To evaluate the expression of voltage-gated potassium channel Kv3.4 and to determine its significance in oral squamous cell carcinoma (OSCC) and precancerous lesions.
Immunohistochemical SP methods were performed to detect the expression of Kv3.4 at tissue level on 57 paraffin-embedded samples collected from Pathology Department of Anhui Province Stomatological Hospital during January 2013 to June 2014. The relationships between the expression of Kv3.4 and precancerous lesion and clinical pathologic factors of oral squamous cell carcinoma, such as pathologic classification, clinical stage and lymph node metastasis, were also analyzed. Totally 6 samples of normal oral mucosa tissue, 23 samples of OSCC and 13 samples of precancerous lesions were collected from the Department of Oral and Maxillofacial Surgery of The First Affiliated Hospital of Anhui Medical University during May 2014 to March 2015. Real-time quantitative PCR and Western blotting were used to detect the expression of Kv3.4 in these 42 samples at molecular and protein levels.
Real-time quantitative PCR showed the relative expression quantity of Kv3.4 in normal oral mucosa, precancerous lesions, and OSCC tissues were 0.85±0.48, 3.50±2.51 and 18.48±7.70, respectively. The relative expression quantity of Kv3.4 in OSCC and precancerous lesions were higher than that in normal group, the differences were both statistically significant (P=0.002, P=0.029). The relative expression quantities of Kv3.4 protein in precancerous and OSCC tissues were 0.87±0.14 and 0.35±0.03 respectively by Western blotting, and both were higher than that in normal tissues (0.18±0.10). The differences were statistically significant (P=0.002). In 57 paraffin-embedded samples, the positive expression rates of Kv3.4 in normal, precancerous and OSCC tissues were 2/6, 13/18 and 95% (37/39), respectively. The differences were statistically significant(P<0.05). However, the expression of Kv3.4 did not show obvious correlation with patients' genders, presence of lymph node metastasis and clinical stages (P>0.05).
The expression of Kv3.4 is positively correlated to OSCC's occurrence and development. Detection of the expression of Kv3.4 may be used for early diagnosis and prognostic judgment of oral squamous cell carcinoma and precancerous lesions.
评估电压门控钾通道Kv3.4的表达情况，并确定其在口腔鳞状细胞癌（OSCC）及癌前病变中的意义。
采用免疫组织化学SP法，对2013年1月至2014年6月期间安徽省口腔医院病理科收集的57例石蜡包埋样本进行组织水平Kv3.4表达检测。分析Kv3.4表达与口腔鳞状细胞癌癌前病变及临床病理因素（如病理分级、临床分期和淋巴结转移）之间的关系。2014年5月至2015年3月期间，从安徽医科大学第一附属医院口腔颌面外科收集6例正常口腔黏膜组织样本、23例OSCC样本和13例癌前病变样本。采用实时定量PCR和蛋白质印迹法检测这42例样本中Kv3.4在分子和蛋白质水平的表达。
实时定量PCR显示，正常口腔黏膜、癌前病变及OSCC组织中Kv3.4的相对表达量分别为0.85±0.48、3.50±2.51和18.48±7.70。OSCC及癌前病变中Kv3.4的相对表达量高于正常组，差异均有统计学意义（P = 0.002，P = 0.029）。蛋白质印迹法检测显示，癌前病变及OSCC组织中Kv3.4蛋白的相对表达量分别为0.87±0.14和0.35±0.03，均高于正常组织（0.18±0.10）。差异有统计学意义（P = 0.002）。在57例石蜡包埋样本中，正常、癌前病变及OSCC组织中Kv3.4的阳性表达率分别为2/6、13/18和95%（37/39）。差异有统计学意义（P<0.05）。然而，Kv3.4的表达与患者性别、淋巴结转移情况及临床分期无明显相关性（P>0.05）。
Kv3.4的表达与OSCC的发生发展呈正相关。检测Kv3.4的表达可用于口腔鳞状细胞癌及癌前病变的早期诊断和预后判断。
