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[外源水杨酸对干旱胁迫下小冠花种子萌发及生理特性的影响。]

[Effects of exogenous salicylic acid on seed germination and physiological characteristics of Coronilla varia under drought stress.].

作者信息

Ma Le Yuan, Chen Nian Lai, Han Guo Jun, Li Liang

机构信息

College of Resources and Environmental Sciences, Gansu Agricultural University, Lanzhou 730070, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2017 Oct;28(10):3274-3280. doi: 10.13287/j.1001-9332.201710.004.

Abstract

This research investigated the effects of different concentrations (0, 0.5, 1.0, 2.0 mmol·L) of salicylic acid on the seed germination and physiological characteristics of legume forage Coronilla varia (cultivar 'Lvbaoshi') under PEG-6000 (concentration 8% and 12%) simulated drought stress. The results showed that under drought stress, 0.5-1.0 mmol·L salicylic acid significantly increased germination percentage, germination vigour, germination index, vitality index and bud length of C. varia. Under the stress of 12% PEG, the dry mass of C. varia seedlings processed by 1.0 mmol·L salicylic acid was significantly higher than that under drought stress. 0.5-1.0 mmol·L salicylic acid processing significantly increased proline, soluble protein content, the activities of catalase, peroxidase and superoxide dismutase of C. varia seedlings under drought stress, but cell electrolyte permeability, H2O2 content and O2 production rate of seedlings were significantly decreased. 1.0 mmol·L salicylic acid produced the best results. When the concentration of salicylic acid was beyond 2.0 mmol·L, no mitigation effect was observed on the seed germination and growth of seedlings under drought stress. It was concluded that salicylic acid at appropriate concentrations could effectively improve osmotic regulation, antioxidation and mitigate the damage of drought stress so as to promote the growth of C. varia seedlings.

摘要

本研究探讨了在聚乙二醇 - 6000(浓度8%和12%)模拟干旱胁迫下,不同浓度(0、0.5、1.0、2.0 mmol·L)水杨酸对豆科牧草变色锦鸡儿(品种‘绿宝石’)种子萌发及生理特性的影响。结果表明,在干旱胁迫下,0.5 - 1.0 mmol·L水杨酸显著提高了变色锦鸡儿的发芽率、发芽势、发芽指数、活力指数和芽长。在12%聚乙二醇胁迫下,1.0 mmol·L水杨酸处理的变色锦鸡儿幼苗干质量显著高于干旱胁迫下的干质量。0.5 - 1.0 mmol·L水杨酸处理显著提高了干旱胁迫下变色锦鸡儿幼苗的脯氨酸、可溶性蛋白含量以及过氧化氢酶、过氧化物酶和超氧化物歧化酶的活性,但幼苗的细胞电解质渗透率、H2O2含量和O2产生速率显著降低。1.0 mmol·L水杨酸处理效果最佳。当水杨酸浓度超过2.0 mmol·L时,在干旱胁迫下对种子萌发和幼苗生长未观察到缓解作用。研究得出结论,适宜浓度的水杨酸能有效改善渗透调节、抗氧化作用,减轻干旱胁迫的伤害,从而促进变色锦鸡儿幼苗生长。

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