三个猪品种的甲基组、转录组和 miRNA 组的综合分析。
Integrated analysis of methylome, transcriptome and miRNAome of three pig breeds.
机构信息
Key Lab of Agriculture Animal Genetics, Breeding, & Reproduction of Ministry of Education, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Institute of Animal Genetics & Breeding, College of Animal Science & Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
出版信息
Epigenomics. 2018 May;10(5):597-612. doi: 10.2217/epi-2017-0087. Epub 2018 Apr 25.
AIM
Integrated analysis of methylome and transcriptome may help understand the molecular basis of the different breeds with different traits of commercial interest.
MATERIALS & METHODS: We obtained the first genome-wide methylome with single-base resolution, miRNAome and transcriptome from three swine breeds.
RESULTS
We displayed the landscape of the three omics in the whole-genome level. Integrated outcomes of methylome with genetic selection, miRNAome and transcriptome are also provided. Finally, we identified 11 candidate differentially methylated genes associated with phenotype variance in pigs.
CONCLUSION
DNA methylation not only suppresses transcriptome but also miRNAome. The different -omics data have complicated interaction in directly or indirectly and exhibited close relations with the distinct phenotypic traits of growth, disease resistance and energy metabolism.
目的
整合分析甲基化组和转录组有助于理解具有不同商业性状的不同品种的分子基础。
材料与方法
我们从三个猪品种中获得了首个具有单碱基分辨率的全基因组甲基化组、miRNA 组和转录组。
结果
我们展示了三个组学在全基因组水平上的图谱。还提供了甲基化组与遗传选择、miRNA 组和转录组的综合结果。最后，我们鉴定了 11 个与猪表型变异相关的候选差异甲基化基因。
结论
DNA 甲基化不仅抑制转录组，还抑制 miRNA 组。不同的组学数据在直接或间接相互作用复杂，与生长、抗病和能量代谢等不同表型性状密切相关。
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