Dubey J P
United States Department of Agriculture,Agricultural Research Service,Animal Parasitic Diseases Laboratory,Beltsville Agricultural Research Center,Beltsville,Maryland 20705-2350,USA.
Parasitology. 2018 Dec;145(14):1845-1852. doi: 10.1017/S0031182018000604. Epub 2018 Apr 25.
Water buffalo (Bubalus bubalis) is important for the economy of Asia, South America and parts of Europe. Coccidiosis is an important cause of neonatal mortality in livestock, including buffalo. Of more than 12 species of Eimeria in buffalo, Eimeria bareillyi is the most pathogenic. There are uncertainties concerning its asexual and sexual development. During a previously reported outbreak of fatal enteritis associated with E. bareillyi in buffaloes in the Netherlands, sections of small intestine were re-evaluated histologically and by transmission electron microscopy (TEM) to seek details of endogenous development. Profuse asexual multiplication occurred in the jejunum and ileum. Light microscopic examination revealed that parasites divided in two (probably endodyogeny) or more organisms. There were two or more generations of morphologically different merozoites; some of these observations were confirmed by TEM. Details of gametogonic development, including oocyst wall formation are provided. Schizogonic and gametogonic development described in the present study can serve as a guide for differential diagnosis of Eimeria species in histological sections of intestines of buffaloes.
水牛（Bubalus bubalis）对亚洲、南美洲和欧洲部分地区的经济具有重要意义。球虫病是包括水牛在内的家畜新生儿死亡的重要原因。在水牛体内超过12种艾美耳球虫中，巴雷利艾美耳球虫（Eimeria bareillyi）致病性最强。其无性和有性发育存在一些不确定性。在荷兰之前报道的一次与巴雷利艾美耳球虫相关的水牛致命性肠炎暴发期间，对小肠切片进行了组织学和透射电子显微镜（TEM）重新评估，以探寻内源性发育的细节。空肠和回肠中发生了大量无性繁殖。光学显微镜检查显示寄生虫分裂为两个（可能是内二分裂）或更多个体。存在两代或更多代形态不同的裂殖子；其中一些观察结果通过透射电子显微镜得到了证实。本文提供了配子生殖发育的细节，包括卵囊壁形成。本研究中描述的裂殖生殖和配子生殖发育可为水牛肠道组织切片中艾美耳球虫种类的鉴别诊断提供指导。
