大西洋鳕鱼（Gadus morhua）血浆蛋白质组研究：暴露于原油引起的变化。
Study of the plasma proteome of Atlantic cod (Gadus morhua): Changes due to crude oil exposure.
机构信息
International Research Institute of Stavanger (IRIS) - Environment Department, Mekjarvik 12, NO-4070 Randaberg, Norway; Faculty of Science and Technology, Department of Chemistry Bioscience and Environmental Engineering, University of Stavanger, NO-4036 Stavanger, Norway.
Faculty of Science and Technology, Department of Chemistry Bioscience and Environmental Engineering, University of Stavanger, NO-4036 Stavanger, Norway.
出版信息
Mar Environ Res. 2018 Jul;138:46-54. doi: 10.1016/j.marenvres.2018.03.009. Epub 2018 Apr 17.
Oil contamination is an environmental issue of great concern and the necessity for background studies and monitoring programs to continuously evaluate the levels of oil pollution is required. In this study, Atlantic cod (Gadus morhua) were exposed to dispersed crude oil for 1 and 4 weeks to simulate environmental contamination. Fractionated plasma samples were then analysed by tandem mass spectrometry. In total, 717 proteins were identified and 10 new protein biomarker candidates were found. The significant proteome changes were related to the immune response by alterations in the levels of specific immunoglobulins, alpha-2-macroglobulin and galectin-3-binding proteins. After 4 weeks of oil exposure, a lowered level of a NLRC3-like protein was also observed. The results from this study provide insight into the Atlantic cod plasma proteome and into the toxicological effects and potential response mechanisms of short and long-term exposure to crude oil.
石油污染是一个备受关注的环境问题，需要进行背景研究和监测计划，以持续评估石油污染水平。在这项研究中，大西洋鳕鱼（Gadus morhua）被暴露于分散的原油中 1 周和 4 周，以模拟环境污染。然后通过串联质谱法分析分馏的血浆样本。总共鉴定出 717 种蛋白质，并发现了 10 种新的蛋白质生物标志物候选物。显著的蛋白质组变化与免疫反应有关，具体表现为特定免疫球蛋白、α-2-巨球蛋白和半乳糖凝集素-3 结合蛋白水平的变化。在暴露于石油 4 周后，还观察到一种 NLRC3 样蛋白的水平降低。这项研究的结果提供了对大西洋鳕鱼血浆蛋白质组的深入了解，以及对短期和长期暴露于原油的毒理学效应和潜在反应机制的了解。
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