Occurrence of genes encoding carbapenem-resistant and from Intensive Care Unit in a tertiary care hospital.

CONTEXT

ICU shows increasing incidence of infection associated with the use of invasive procedures for the diagnostic purpose as well as the indiscriminate use of antibiotics. and are "very successful" pathogen and the emergence of the Metallo-β-Lactamases (MBL) is becoming a therapeutic challenge.

AIMS

To isolate the Gram negative bacilli from the ICU samples. To identify the metallo betalactamase producers and to detect the gene presence among the and .

SETTINGS AND DESIGN

The Nonfermenting Gram negative bacilli isolates from the ICU samples were taken over for 5 years (2009-2014) in a tertiary care hospital.

METHODS AND MATERIALS

The isolates of and were confirmed by API analyser and processed according to standard procedures. Detection of the MBL producers were done by E strip method and subjected for gene detection by PCR method.

RESULTS

In our study a total of 195 isolates of were obtained from various ICU. Of these MBL producers, 26 % were and 25 % were . The subtypes of MBL producing were 26%. The predominant gene coding for MBL activity in were found to be gene 11.9%. The gene accession numbers were KF975367, KF975372.

CONCLUSIONS

We have to control the development and dissemination of these superbugs among the ICU's.