Yamada Kenichi, Hiradate Yuuki, Goto Mei, Nishiyama Chiho, Hara Kenshiro, Yoshida Hiroaki, Tanemura Kentaro
Laboratory of Animal Reproduction and Development Graduate School of Agricultural Science Tohoku University Sendai Japan.
Sendai ART Clinic Sendai Japan.
Reprod Med Biol. 2018 Feb 3;17(2):143-148. doi: 10.1002/rmb2.12079. eCollection 2018 Apr.
As disturbed mitochondrial distribution is thought to be a cause of the aging of oocytes, it was investigated whether oxidizing agents exert harmful effects on nuclear maturation and mitochondrial cluster formation in murine oocytes and whether antioxidants could rescue such harmful effects in vitro.
Oocytes were obtained from female Institute of Cancer Research mice 48 h after an intraperitoneal injection of 7.5 IU pregnant mare serum gonadotropin. The oocytes were cultured with potassium bromate, an oxidizing agent, in the presence or absence of the antioxidant, resveratrol. After 12 h, the nuclear phases and mitochondrial distribution were observed.
Significantly decreased rates of metaphase II (MII) oocytes were observed with 750 μM and 1000 μM of potassium bromate, while a significant increase in abnormal mitochondrial clusters was induced at 500 μM, 750 μM, and 1,000 μM. The addition of 10 μM or 20 μM resveratrol improved both MII maturity and the cluster formation rates in the presence of potassium bromate.
The addition of potassium bromate reduced MII maturity rates and induced abnormal mitochondrial cluster formation. This effect was alleviated by the antioxidant, resveratrol. The in vitro model used herein is useful for investigating the functions of antioxidants in the aging of oocytes.
由于线粒体分布紊乱被认为是卵母细胞衰老的一个原因，因此研究了氧化剂是否对小鼠卵母细胞的核成熟和线粒体聚集形成产生有害影响，以及抗氧化剂是否能在体外挽救这种有害影响。
在腹腔注射7.5国际单位孕马血清促性腺激素48小时后，从癌症研究所雌性小鼠获取卵母细胞。将卵母细胞与氧化剂溴酸钾一起培养，同时存在或不存在抗氧化剂白藜芦醇。12小时后，观察核相和线粒体分布。
观察到750μM和1000μM溴酸钾处理后，中期II（MII）卵母细胞的比例显著降低，而在500μM、750μM和1000μM时，异常线粒体聚集显著增加。在存在溴酸钾的情况下，添加10μM或20μM白藜芦醇可提高MII成熟率和聚集形成率。
添加溴酸钾降低了MII成熟率并诱导了异常线粒体聚集形成。抗氧化剂白藜芦醇减轻了这种影响。本文使用的体外模型有助于研究抗氧化剂在卵母细胞衰老中的作用。
