单克隆抗体DH12与人纤连蛋白受体β亚基的细胞表面形式和前体形式发生反应。

Monoclonal antibody DH12 reacts with a cell surface and a precursor form of the beta subunit of the human fibronectin receptor.

作者信息

De Strooper B, Saison M, Jaspers M, Spaepen M, Van Leuven F, Van den Berghe H, Cassiman J J

机构信息

Center for Human Genetics, University of Leuven, Belgium.

出版信息

Cell Biol Int Rep. 1988 Jan;12(1):9-16. doi: 10.1016/0309-1651(88)90106-3.

DOI:10.1016/0309-1651(88)90106-3

PMID:2969294

Abstract

Monoclonal and polyclonal antibodies were raised against a placenta plasma membrane protein preparation, which was obtained by fractionation on Blue B dye matrix and by HPLC-anionexchange, and which was shown to contain fibronectin receptors. Immunochemical and functional evidence showed that monoclonal antibody DH12 recognized the beta subunit of the human fibronectin receptor on fibroblasts. This monoclonal antibody reacted with two proteins in Western blots and in double immune precipitations of whole cell preparations. Only the higher Mr protein became labeled by surface iodination of intact fibroblasts. The lower Mr protein is thought to be an intracellular precursor of the beta subunit of the fibronectin receptor.

摘要

针对一种胎盘质膜蛋白制剂制备了单克隆抗体和多克隆抗体，该制剂是通过在蓝色B染料基质上分级分离以及高效液相色谱阴离子交换获得的，并且已证明其含有纤连蛋白受体。免疫化学和功能证据表明，单克隆抗体DH12识别成纤维细胞上人类纤连蛋白受体的β亚基。这种单克隆抗体在蛋白质印迹法以及全细胞制剂的双重免疫沉淀中与两种蛋白质发生反应。只有分子量较高的蛋白质在完整成纤维细胞的表面碘化过程中被标记。分子量较低的蛋白质被认为是纤连蛋白受体β亚基的细胞内前体。