人纤连蛋白受体β亚基基因定位于10号染色体。

Mapping of human fibronectin receptor beta subunit gene to chromosome 10.

作者信息

Zhang Y, Saison M, Spaepen M, De Strooper B, Van Leuven F, David G, Van den Berghe H, Cassiman J J

机构信息

Center for Human Genetics, University of Leuven, Belgium.

出版信息

Somat Cell Mol Genet. 1988 Jan;14(1):99-104. doi: 10.1007/BF01535053.

DOI:10.1007/BF01535053

PMID:2963381

Abstract

Human-mouse hybrid cells were examined by indirect immunofluorescence with Mab DH12, a monoclonal antibody that recognizes the beta subunit of the human fibronectin receptor. Cells that expressed the antigen at their surface were sorted by FACS and karyotyped. Immunoaffinity chromatography on Mab DH12 was used to confirm the presence of the human antigen. The chromosome assignment was strengthened by isozyme analysis of markers for chromosomes 9 and 10. The results are suggestive for a 10p mapping of this beta subunit of the fibronectin receptor. Since the gene coding for the beta subunit of the VLA proteins was previously assigned to the same chromosome, our result could provide further evidence for the relationship between the beta subunit of the human fibronectin receptor and the VLA protein family.

摘要

用人源小鼠杂交细胞与单克隆抗体Mab DH12进行间接免疫荧光检测，Mab DH12是一种识别人类纤连蛋白受体β亚基的单克隆抗体。通过荧光激活细胞分选术（FACS）对表面表达该抗原的细胞进行分选并进行核型分析。利用Mab DH12进行免疫亲和层析以确认人类抗原的存在。通过对9号和10号染色体标记物的同工酶分析，强化了染色体定位。结果提示纤连蛋白受体的该β亚基定位于10p。由于之前已将编码VLA蛋白β亚基的基因定位于同一条染色体，我们的结果可为人类纤连蛋白受体β亚基与VLA蛋白家族之间的关系提供进一步证据。