Annapurna Kannepalli, Govindasamy Venkadasamy, Sharma Meenakshi, Ghosh Arpita, Chikara Surendra K
1Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
Eurofins Genomics India Private Limited, Bangalore, 560048 Karnataka India.
3 Biotech. 2018 May;8(5):223. doi: 10.1007/s13205-018-1242-y. Epub 2018 Apr 20.
strain KMS 80 (MTCC No. 12704) is an isolate from the root tissues of rice ( L.) that displays biological nitrogen fixation and plant growth promoting abilities. Here, we report the complete genome sequence of this strain, which contains 4,566,040 bp, 4424 protein-coding genes, 8692 promoter sequences, 67 tRNAs, 20 rRNA genes with six copies of 5S rRNAs along with a single copy of 16S-23S rRNA and genome average GC-content of 45.50%. Twenty one genes involved in nitrogen metabolism pathway and two main transcriptional factor genes, and responsible for regulation of nitrogen fixation in sp. were predicted from the whole genome of strain KMS 80. Analysis of the ~ 4.57 Mb genome sequence will give support to understand the genetic determinants of host range, endophytic colonization behaviour as well as to enhance endophytic nitrogen fixation and other plant beneficial role of in rice.
菌株KMS 80（MTCC编号12704）是从水稻（L.）根组织中分离得到的，具有生物固氮和促进植物生长的能力。在此，我们报告该菌株的完整基因组序列，其包含4566040 bp，4424个蛋白质编码基因，8692个启动子序列，67个tRNA，20个rRNA基因（其中5S rRNA有6个拷贝，16S - 23S rRNA有1个拷贝），基因组平均GC含量为45.50%。从菌株KMS 80的全基因组中预测出21个参与氮代谢途径的基因以及两个主要的转录因子基因， 负责调控 种中的固氮作用。对约4.57 Mb基因组序列的分析将有助于理解宿主范围、内生定殖行为的遗传决定因素，以及增强 在水稻中的内生固氮和其他对植物有益的作用。
