Department of Gynaecological Oncology and Gynaecology, Medical University of Lublin, 20‑081 Lublin, Poland.
Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20‑033 Lublin, Poland.
Mol Med Rep. 2018 Jun;17(6):8253-8259. doi: 10.3892/mmr.2018.8909. Epub 2018 Apr 20.
From a clinical point of view, easily obtainable and useful markers of a particular pathological status are required for appropriate diagnosis and treatment. Analysis of the proteomic profile of saliva may allow for the selection of potential marker of preterm delivery in humans. Saliva samples were collected from 12 patients diagnosed with threatened preterm delivery and 10 controls with uncomplicated pregnancies at the same gestational age. Samples were analysed using 2D electrophoresis. Based on statistical analysis, spots of interest were selected and collected from gels. Subsequently, spots were decoloured and proteins were identified by mass spectrometry using the matrix assisted laser desorption ionization‑time of flight technique. The results of identification were compared with the Swiss‑Prot database. A total of 1,393 spots were detected in the present study with 59 significantly different between control and preterm samples. Increased intensity of staining of 32 spots was observed in the premature delivery group compared with control patients and 27 spots were stained more intensely in the control group compared with the premature delivery group. A total of nine spots, which were significantly different between examined samples were identified and three of them exhibited increased intensity of staining in premature delivery group compared with controls, including dedicator of cytokinesis protein 1, metallothionein‑2, guanylyl cyclase‑activating protein 1. The six remaining spots included, epithelial‑stromal interaction protein 1, serum albumin, tyrosine‑tRNA ligase, cytoplasmic, protein chibby homolog 3, leukemia inhibitory factor receptor and adenosylhomocysteinase 3, and exhibited increased intensity of staining in healthy controls compared with premature delivery group. Further studies with an increased number of patients and identification of the complete protein profile are required to confirm the results of the present study and applicability of saliva as a source of disease biomarkers.
从临床角度来看，需要容易获得且有用的特定病理状态标志物来进行适当的诊断和治疗。分析唾液的蛋白质组谱可能允许选择人类早产的潜在标志物。从 12 名被诊断为先兆早产的患者和 10 名同期妊娠无并发症的对照者中采集唾液样本。使用二维电泳分析样本。基于统计学分析，从凝胶中选择并收集有意义的斑点。随后，斑点脱色并用基质辅助激光解吸电离-飞行时间质谱技术进行质谱鉴定。鉴定结果与 Swiss-Prot 数据库进行比较。本研究共检测到 1393 个斑点，其中对照组和早产组之间有 59 个斑点存在显著差异。与对照组相比，早产组中 32 个斑点的染色强度增加，而对照组中 27 个斑点的染色强度增加。共鉴定出 9 个斑点，在实验组和对照组之间有显著差异，其中 3 个斑点在早产组中的染色强度高于对照组，包括细胞分裂蛋白 1、金属硫蛋白 2 和鸟苷酸环化酶激活蛋白 1。其余 6 个斑点包括上皮-基质相互作用蛋白 1、血清白蛋白、酪氨酸-tRNA 连接酶、细胞质、类 Chibby 同源蛋白 3、白血病抑制因子受体和腺苷同型半胱氨酸酶 3，与早产组相比，在健康对照组中，这些斑点的染色强度增加。需要进一步的研究，增加患者数量并鉴定完整的蛋白质谱，以确认本研究的结果和唾液作为疾病生物标志物来源的适用性。
