Jiangsu Key Laboratory for Microbes and Microbial Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science , Nanjing Normal University , Nanjing 210023 , PR China.
J Agric Food Chem. 2018 May 9;66(18):4702-4709. doi: 10.1021/acs.jafc.8b01343. Epub 2018 Apr 30.
Coprinopsis polysaccharides exhibit hypoglycemic and antioxidant activities. In this report, increases in polysaccharide production by homologous co-overexpression or individual homologous overexpression of phosphoglucomutase and UDP glucose pyrophosphorylase gene in Coprinopsis cinerea, which participate in polysaccharide biosynthesis. The transcription levels of the target genes were upregulated significantly in the oePGM-UGP strain when compared with the oePGM or oeUGP strain. The maximum intracellular polysaccharide content obtained in the oePGM-UGP strain was 1.49-fold higher than that of the WT strain, whereas a slight improvement in polysaccharide production was obtained in the oePGM and oeUGP strains. Extracellular polysaccharide production was enhanced by 75% in the oePGM-UGP strain when compared with that of the WT strain, whereas improvements of 30% and 16% were observed for the oePGM and oeUGP strains, respectively. These results show that multiple interventions in polysaccharide biosynthesis pathways of Basidiomycetes might improve polysaccharide yields when compared with that of single interventions.
毛头鬼伞多糖具有降血糖和抗氧化活性。在本报告中，通过同源共过表达或磷酸葡萄糖变位酶和 UDP 葡萄糖焦磷酸化酶基因的单独同源过表达，毛头鬼伞多糖的产量增加，这两个基因参与多糖的生物合成。与 oePGM 或 oeUGP 菌株相比，oePGM-UGP 菌株中靶基因的转录水平显著上调。oePGM-UGP 菌株的最大胞内多糖含量比 WT 菌株高 1.49 倍，而 oePGM 和 oeUGP 菌株的多糖产量略有提高。与 WT 菌株相比，oePGM-UGP 菌株的胞外多糖产量提高了 75%，而 oePGM 和 oeUGP 菌株分别提高了 30%和 16%。这些结果表明，与单一干预相比，双相干预担子菌多糖生物合成途径可能提高多糖产量。
