Wang Qiong, Xu Mengmeng, Zhao Liting, Chen Lei, Ding Zhongyang
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Microorganisms. 2023 Mar 17;11(3):772. doi: 10.3390/microorganisms11030772.
Polysaccharides are crucial dietary supplements and traditional pharmacological components of ; however, the mechanisms responsible for high polysaccharide yields in remain unclear. Therefore, we investigated the mechanisms underlying the high yield of polysaccharides in submerged cultures of using transcriptomic and proteomic analyses. Several glycoside hydrolase (GH) genes and proteins, which are associated with the degradation of fungal cell walls, were significantly upregulated under high polysaccharide yield conditions. They mainly belonged to the GH3, GH5, GH16, GH17, GH18, GH55, GH79, GH128, GH152, and GH154 families. Additionally, the results suggested that the cell wall polysaccharide could be degraded by GHs, which is beneficial for extracting more intracellular polysaccharides from cultured mycelia. Furthermore, some of the degraded polysaccharides were released into the culture broth, which is beneficial for obtaining more extracellular polysaccharides. Our findings provide new insights into the mechanisms underlying the roles that GH family genes play to regulate high polysaccharide yields in .
多糖是重要的膳食补充剂和传统药理成分；然而，导致[具体对象]中多糖高产的机制仍不清楚。因此，我们利用转录组学和蛋白质组学分析方法，研究了[具体对象]深层培养中多糖高产的潜在机制。在多糖高产条件下，几个与真菌细胞壁降解相关的糖苷水解酶（GH）基因和蛋白质显著上调。它们主要属于GH3、GH5、GH16、GH17、GH18、GH55、GH79、GH128、GH152和GH154家族。此外，结果表明细胞壁多糖可被GHs降解，这有利于从培养菌丝体中提取更多的细胞内多糖。此外，一些降解的多糖被释放到培养液中，这有利于获得更多的细胞外多糖。我们的研究结果为GH家族基因在调节[具体对象]多糖高产中所起作用的潜在机制提供了新的见解。
