Franco-Martínez Lorena, Romero Diego, Rubio Camila Peres, Tecles Fernando, Martínez-Subiela Silvia, Teles Mariana, Tvarijonaviciute Asta
Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, Murcia 30100, Spain.
Department of Toxicology, Regional Campus of International Excellence Mare Nostrum, University of Murcia, Espinardo, E-30071, Murcia 30100, Spain.
Comp Biochem Physiol B Biochem Mol Biol. 2018 Jul;221-222:44-49. doi: 10.1016/j.cbpb.2018.04.006. Epub 2018 Apr 23.
The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). The assays were precise, accurate and provided low limits of detection. When oxidative stress was promoted by inducing hypoxia and the behaviour of these biomarkers between hypoxic and controls mussels were compared, statistically significant differences were observed in all biomarkers and tissues evaluated. The results of the present study demonstrated that these biomarkers, not previously studied in mussels, show a potential use as biomarkers of oxidative stress in this species since they were validated and showed changes under a state of oxidative stress.
本报告的目的是开发并验证用于测量贻贝鳃、消化腺和血淋巴样本中总抗氧化能力（TAC）、硫醇和晚期氧化蛋白产物（AOPP）的新型自动分光光度法，并评估其在生物监测项目中的潜在用途。通过不同方法测量总抗氧化能力（TAC）：特洛克斯等效抗氧化能力（TEAC1和TEAC2）、铜离子还原抗氧化能力（CUPRAC）和血浆铁还原能力（FRAP）。这些测定方法精确、准确且检测限低。当通过诱导缺氧来促进氧化应激并比较缺氧贻贝和对照贻贝之间这些生物标志物的行为时，在所有评估的生物标志物和组织中均观察到统计学上的显著差异。本研究结果表明，这些先前未在贻贝中研究过的生物标志物，显示出作为该物种氧化应激生物标志物的潜在用途，因为它们经过了验证并在氧化应激状态下表现出变化。
