Rubio Camila Peres, Contreras-Aguilar Maria Dolores, Quiles Alberto, López-Arjona Marina, Cerón José Joaquin, Martínez-Subiela Silvia, Hevia Maria Luisa, Escribano Damián, Tecles Fernando
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Espinardo, Murcia, Spain.
Department of Animal Production, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Espinardo, Murcia, Spain.
Res Vet Sci. 2019 Apr;123:71-76. doi: 10.1016/j.rvsc.2018.12.015. Epub 2018 Dec 22.
Oxidative stress can affect animal's health and the quality of its final products. The oxidative status can be evaluated by the measurement of both oxidant and antioxidant biomarkers. The use of saliva as a sample is preferable to blood, as individuals with limited training can collect it easily and non-invasively with minimal stress to the animal. The aim of this study was to perform an analytical validation of automated assays of the ferric reducing ability of plasma (FRAP), the cupric reducing antioxidant capacity (CUPRAC), the Trolox equivalent antioxidant capacity (TEAC) and uric acid as antioxidant biomarkers and of the advanced oxidation protein products (AOPP) and hydrogen peroxide (HO) as oxidant biomarkers in saliva samples of sheep, and to evaluate their possible changes after stress induced by shearing. All assays produced acceptable results in the analytical validation, from which it can be concluded that oxidative stress biomarkers such as FRAP, CUPRAC, TEAC, uric acid and AOPP and HO can be measured in sheep saliva. In addition, acute stress due to shearing could produce an oxidative stress response in sheep and subsequently increase antioxidants in order to protect cells from damage.
氧化应激会影响动物健康及其最终产品的质量。氧化状态可通过测量氧化剂和抗氧化生物标志物来评估。使用唾液作为样本比血液更可取，因为未经多少训练的个体就能轻松、无创地采集唾液，且对动物造成的应激最小。本研究的目的是对绵羊唾液样本中作为抗氧化生物标志物的血浆铁还原能力（FRAP）、铜还原抗氧化能力（CUPRAC）、特洛克斯等效抗氧化能力（TEAC）和尿酸以及作为氧化剂生物标志物的晚期氧化蛋白产物（AOPP）和过氧化氢（HO）的自动化检测方法进行分析验证，并评估剪毛诱导的应激后它们可能发生的变化。所有检测方法在分析验证中均产生了可接受的结果，由此可以得出结论，FRAP、CUPRAC、TEAC、尿酸、AOPP和HO等氧化应激生物标志物可在绵羊唾液中进行测量。此外，剪毛引起的急性应激可能会在绵羊体内产生氧化应激反应，随后增加抗氧化剂以保护细胞免受损伤。
