Huebner K, Cannizzaro L A, Frey A Z, Hecht B K, Hecht F, Croce C M, Wallner B P
Wistar Institute, Philadelphia, PA 19104.
Oncogene Res. 1988 May;2(4):299-310.
The human genes which code for Lipocortin I and Lipocortin II, proteins that inhibit phospholipase A2 (PLA2) activity, have been regionally localized in the human genome by chromosomal in situ hybridization and segregation analysis in somatic cell hybrids using cDNA clones for Lipocortin I and II. Lipocortin I, the 35 kd substrate for the epidermal growth factor (EGF) receptor/kinase, maps to chromosome region 9q11- greater than q22. The Lipocortin II cDNA probe detects at least four independently segregating loci which map to human chromosome regions 4q21-q31.1, 9pter-q34 proximal to c-abl, 10q proximal to 10q24 and 15q21-q22 proximal to the 15q22 translocation breakpoint characteristic of acute promyelocytic leukemia (APL). Thus, Lipocortin I and one locus detected by Lipocortin II cDNA are syntenic on chromosome 9; one Lipocortin II locus is perhaps not far from the genes for EGF and IL-2 on 4q; and another of the Lipocortin II loci is on 15q, perhaps not far from the APL breakpoint.
编码脂皮质素I和脂皮质素II的人类基因所产生的蛋白质可抑制磷脂酶A2（PLA2）的活性。利用脂皮质素I和II的cDNA克隆，通过染色体原位杂交和体细胞杂种中的分离分析，已将这些基因在人类基因组中进行了区域定位。脂皮质素I是表皮生长因子（EGF）受体/激酶的35kd底物，定位于染色体区域9q11至大于q22。脂皮质素II cDNA探针检测到至少四个独立分离的基因座，它们分别定位于人类染色体区域4q21 - q31.1、9pter - q34（靠近c - abl）、10q（靠近10q24）以及15q21 - q22（靠近急性早幼粒细胞白血病（APL）特征性的15q22易位断点）。因此，脂皮质素I和脂皮质素II cDNA检测到的一个基因座在9号染色体上是同线的；脂皮质素II的一个基因座可能离4q上的EGF和IL - 2基因不远；脂皮质素II的另一个基因座在15q上，可能离APL断点不远。
