Beyene Desta A, Kanarek Norma F, Naab Tammey J, Ricks-Santi Luisel L, Hudson Tamaro S
Department of Biochemistry and Molecular Biology, Howard University, Washington, DC, USA.
Department of Research, Veteran Affairs Medical Center, Washington, DC, USA.
Heliyon. 2020 Feb 5;6(2):e03241. doi: 10.1016/j.heliyon.2020.e03241. eCollection 2020 Feb.
A review of literature on the expression of Annexin 2 in cancer has shown that there is very limited research work on the association of this protein with breast cancer aggressiveness in African Americans. In the present study, TMA breast tissues from African American women were stained with Annexin 2 antibody to determine the association between the molecular subtypes and Annexin 2 protein expression.
An annotated case series of 135 breast cancer tissues archived from 2000 to 2010 was acquired from the Howard University Tumor Registry. The association between ANX2 expression and survival by molecular subtypes Luminal A, Luminal B, HER2, and Triple Negative (TN) was assessed using Multinomial regression, chi-square analysis, and Kaplan-Meir graphs (Stata 11).
Our findings show a marked association between ANX2 protein expression in Luminal B and HER2 subtypes unadjusted and when adjusted for age. Borderline differences in tumor grade were found in TN only.Univariately, age (<50, 50 + years) and metastases were highly significant for overall survival, disease-free survival and recurrence-free survival. Stage, tumor size, and nodal involvement were of borderline or greater significance for overall and disease-free survival. ANX2 expression was not significant. Kaplan Meier tests of ANX2 showed significant separation of overall survival by ANX2 protein expression in all breast tumor subtypes. In multivariate analyses comparing TN to Luminal A, ANX2 was not important while controlling for age and grade.
ANX2 might be a biomarker of aggressiveness and a relevant candidate biomarker in high risk African American women with Luminal B and HER2 breast cancer.
对有关膜联蛋白2在癌症中表达的文献综述表明，关于该蛋白与非裔美国人乳腺癌侵袭性之间关联的研究工作非常有限。在本研究中，用膜联蛋白2抗体对非裔美国女性的组织微阵列（TMA）乳腺组织进行染色，以确定分子亚型与膜联蛋白2蛋白表达之间的关联。
从霍华德大学肿瘤登记处获取了135例2000年至2010年存档的乳腺癌组织的注释病例系列。使用多项回归、卡方分析和Kaplan-Meier图（Stata 11）评估膜联蛋白2（ANX2）表达与分子亚型管腔A型（Luminal A）、管腔B型（Luminal B）、人表皮生长因子受体2型（HER2）和三阴性（TN）之间的生存关联。
我们的研究结果显示，在未调整以及调整年龄后，管腔B型和HER2亚型中ANX2蛋白表达之间存在显著关联。仅在三阴性乳腺癌中发现肿瘤分级存在临界差异。单因素分析中，年龄（<50岁、50岁及以上）和转移对总生存期、无病生存期和无复发生存期具有高度显著意义。分期（阶段）、肿瘤大小和淋巴结受累对总生存期和无病生存期具有临界或更大的意义。ANX2表达无显著意义。对ANX2进行的Kaplan Meier检验显示，在所有乳腺肿瘤亚型中，ANX2蛋白表达对总生存期有显著区分。在将三阴性乳腺癌与管腔A型进行比较的多变量分析中，在控制年龄和分级时，ANX2并不重要。
ANX2可能是侵袭性的生物标志物，并且是高危非裔美国管腔B型和HER2型乳腺癌女性中一个相关的候选生物标志物。
