Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu, 808-0196, Japan.
CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China.
Appl Microbiol Biotechnol. 2018 Jun;102(12):5323-5334. doi: 10.1007/s00253-018-9003-8. Epub 2018 Apr 25.
Efficient approaches for the utilization of waste sewage sludge have been widely studied. One of them is to use it for the bioenergy production, specifically methane gas which is well-known to be driven by complex bacterial interactions during the anaerobic digestion process. Therefore, it is important to understand not only microorganisms for producing methane but also those for controlling or regulating the process. In this study, azithromycin analogs belonging to macrolide, ketolide, and lincosamide groups were applied to investigate the mechanisms and dynamics of bacterial community in waste sewage sludge for methane production. The stages of anaerobic digestion process were evaluated by measuring the production of intermediate substrates, such as protease activity, organic acids, the quantification of bacteria and archaea, and its community dynamics. All azithromycin analogs used in this study achieved a high methane production compared to the control sample without any antibiotic due to the efficient hydrolysis process and the presence of important fermentative bacteria and archaea responsible in the methanogenesis stage. The key microorganisms contributing to the methane production may be Clostridia, Cladilinea, Planctomycetes, and Alphaproteobacteria as an accelerator whereas Nitrosomonadaceae and Nitrospiraceae may be suppressors for methane production. In conclusion, the utilization of antibiotic analogs of macrolide, ketolide, and lincosamide groups has a promising ability in finding the essential microorganisms and improving the methane production using waste sewage sludge.
已广泛研究了有效利用废污水污泥的方法。其中之一是将其用于生物能源生产，特别是甲烷气体，众所周知，在厌氧消化过程中，甲烷气体是由复杂的细菌相互作用驱动的。因此，了解不仅产生甲烷的微生物，而且控制或调节该过程的微生物也很重要。在这项研究中，应用属于大环内酯、酮内酯和林可酰胺组的阿奇霉素类似物来研究废污水污泥中用于甲烷生产的细菌群落的机制和动态。通过测量中间底物（如蛋白酶活性、有机酸、细菌和古菌的定量及其群落动态）的产生来评估厌氧消化过程的阶段。与没有任何抗生素的对照样品相比，本研究中使用的所有阿奇霉素类似物都由于高效的水解过程以及负责产甲烷阶段的重要发酵细菌和古菌的存在，实现了高甲烷产量。可能有助于甲烷生产的关键微生物可能是梭菌、Cladilinea、浮霉菌和α变形菌，而亚硝化单胞菌科和硝化螺旋菌科可能是甲烷生产的抑制剂。总之，大环内酯、酮内酯和林可酰胺类抗生素类似物的利用具有发现必需微生物和提高利用废污水污泥生产甲烷的潜力。
