Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan.
Department of Plant Pathology, Taiwan Agricultural Research Institute, Taichung, Taiwan.
PLoS One. 2018 Apr 26;13(4):e0196520. doi: 10.1371/journal.pone.0196520. eCollection 2018.
Potato common scab, which is caused by soil-borne Streptomyces species, is a severe plant disease that results in a significant reduction in the economic value of potatoes worldwide. Due to the lack of efficacious pesticides, crop rotations, and resistant potato cultivars against the disease, we investigated whether biological control can serve as an alternative approach. In this study, multiple Bacillus species were isolated from healthy potato tubers, and Bacillus amyloliquefaciens Ba01 was chosen for further analyses based on its potency against the potato common scab pathogen Streptomyces scabies. Ba01 inhibited the growth and sporulation of S. scabies and secreted secondary metabolites such as surfactin, iturin A, and fengycin with potential activity against S. scabies as determined by imaging mass spectrometry. In pot assays, the disease severity of potato common scab decreased from 55.6 ± 11.1% (inoculated with S. scabies only) to 4.2 ± 1.4% (inoculated with S. scabies and Ba01). In the field trial, the disease severity of potato common scab was reduced from 14.4 ± 2.9% (naturally occurring) to 5.6 ± 1.1% after Ba01 treatment, representing evidence that Bacillus species control potato common scab in nature.
马铃薯普通疮痂病是一种由土壤传播的链霉菌引起的严重植物病害，导致全球马铃薯的经济价值显著降低。由于缺乏有效的农药、轮作和对这种疾病具有抗性的马铃薯品种，我们研究了生物防治是否可以作为一种替代方法。在这项研究中，从健康的马铃薯块茎中分离出了多种芽孢杆菌属物种，并根据其对马铃薯普通疮痂病原菌疮痂链霉菌的抑制作用，选择了芽孢杆菌属的 B. amyloliquefaciens Ba01 进行进一步分析。Ba01 抑制了 S. scabies 的生长和孢子形成，并分泌了表面活性剂、伊枯草菌素 A 和丰原素等次级代谢产物，通过成像质谱分析表明它们对 S. scabies 具有潜在的活性。在盆栽试验中，马铃薯普通疮痂病的严重程度从 55.6±11.1%（仅接种 S. scabies）降低到 4.2±1.4%（接种 S. scabies 和 Ba01）。在田间试验中，马铃薯普通疮痂病的严重程度从 14.4±2.9%（自然发生）降低到 Ba01 处理后的 5.6±1.1%，这表明芽孢杆菌属物种在自然条件下控制了马铃薯普通疮痂病。
