Department of Internal Medicine, Chungnam National University Hospital, Daejeon, Republic of Korea.
Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Republic of Korea.
Obesity (Silver Spring). 2018 Jun;26(6):1008-1016. doi: 10.1002/oby.22188. Epub 2018 Apr 26.
The clinical implications of human brown adipose tissue (BAT) were investigated based on the analysis of cervical adipose tissue gene expression under normal physiological conditions.
Matched-pair specimens of adipose tissue (AT) were collected from beneath the incision plane (subcutaneous AT) and from the area surrounding the carotid sheath (carotid AT) from 60 patients undergoing thyroidectomy. The mRNA expression of BAT-associated genes in these tissues was examined, and this expression was correlated with the clinical characteristics of the subjects.
The UCP1 mRNA level was significantly higher in the carotid AT than in the subcutaneous AT. There was an inverse correlation between subject age and the ratio of UCP1 mRNA expression in the carotid AT relative to the subcutaneous AT, which is a measure of BAT activity (r = -0.459; P = 0.004), and there was a negative correlation between BMI and the ratio of UCP1 mRNA expression in subjects with higher BAT activity (r = -0.532; P = 0.016).
UCP1 was identified as the only marker of cervical BAT in humans. There was a negative correlation between obesity and BAT activity in subjects with higher BAT activity, although BAT activity decreased with age.
基于正常生理条件下颈脂组织基因表达的分析，探讨人类棕色脂肪组织（BAT）的临床意义。
从 60 例行甲状腺切除术的患者的切口平面下方（皮下脂肪组织）和颈动脉鞘周围区域（颈动脉脂肪组织）采集配对的脂肪组织（AT）标本。检查这些组织中与 BAT 相关的基因的 mRNA 表达，并将其与受试者的临床特征相关联。
UCP1 mRNA 水平在颈动脉 AT 中明显高于皮下 AT。受试者年龄与颈动脉 AT 中 UCP1 mRNA 表达相对于皮下 AT 的比值呈负相关，这是 BAT 活性的一个衡量指标（r=-0.459；P=0.004），而 BMI 与 BAT 活性较高者的 UCP1 mRNA 表达比值呈负相关（r=-0.532；P=0.016）。
UCP1 被鉴定为人类颈 BAT 的唯一标志物。在 BAT 活性较高的受试者中，肥胖与 BAT 活性呈负相关，尽管 BAT 活性随年龄下降。
