Clinical Research Institute, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310014, Zhejiang, PR China.
Departments of Gastrointestinal and Pancreatic Surgery, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310014, PR China; Key Laboratory of Gastroenterology of Zhejiang Province, Hangzhou 310014, PR China.
Hum Pathol. 2018 Aug;78:44-53. doi: 10.1016/j.humpath.2018.04.008. Epub 2018 Apr 23.
To investigate the expression and clinical significance of long noncoding RNA (lncRNA) in gastric cancer, we applied microarray analysis to obtain expression profiles of protein-coding genes and lncRNAs in tumor and paired adjacent nontumor tissues. We found that 41 lncRNAs were up-regulated and 31 lncRNAs were down-regulated more than 2-fold in gastric cancer versus noncancerous tissues (ratio >2.0, P < .01). We established a coexpression network of the differentially expressed lncRNAs and targeted coding genes that included 17 lncRNAs and 16 coding genes. Because the results of microarray analysis showed that lncRNA M26317 was up-regulated in gastric cancer tissues, we examined the expression level of M26317 in 103 gastric cancer tissues by reverse-transcription polymerase chain reaction and 436 gastric cancer tissues by in situ hybridization. Our data confirmed that M26317 was up-regulated in gastric cancer tissues. Moreover, expression of M26317 correlated with patient age, size of tumor, Lauren's classification, depth of invasion, lymph node and distant metastasis, TNM stage, and poor prognosis (P < .05), but was not associated with sex, location of tumor, and differentiation (P > .05). M26317 may have an important role in malignant transformation and metastasis of gastric cancer.
为了研究长链非编码 RNA（lncRNA）在胃癌中的表达和临床意义，我们应用微阵列分析获得了肿瘤和配对相邻非肿瘤组织中编码蛋白基因和 lncRNA 的表达谱。我们发现，与非癌组织相比，胃癌中 41 个 lncRNA 上调，31 个 lncRNA 下调超过 2 倍（比值>2.0，P<0.01）。我们建立了差异表达 lncRNA 和靶向编码基因的共表达网络，其中包括 17 个 lncRNA 和 16 个编码基因。由于微阵列分析的结果表明 lncRNA M26317 在胃癌组织中上调，我们通过逆转录聚合酶链反应检测了 103 例胃癌组织和 436 例胃癌组织中 M26317 的表达水平。我们的数据证实 M26317 在胃癌组织中上调。此外，M26317 的表达与患者年龄、肿瘤大小、Lauren 分类、浸润深度、淋巴结和远处转移、TNM 分期和预后不良相关（P<0.05），但与性别、肿瘤位置和分化无关（P>0.05）。M26317 可能在胃癌的恶性转化和转移中具有重要作用。
