Xu Chunjing, Shao Yongfu, Xia Tian, Yang Yunben, Dai Jiawei, Luo Lin, Zhang Xinjun, Sun Weiliang, Song Haojun, Xiao Bingxiu, Guo Junming
Department of Biochemistry and Molecular Biology, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, 315211, China.
Tumour Biol. 2014 Oct;35(10):9701-6. doi: 10.1007/s13277-014-2274-5. Epub 2014 Jun 27.
Long noncoding RNAs (lncRNAs) play an important role in cancer occurrence and development. However, there is largely unknown about lncRNAs significance in the diagnosis and treatment of gastric cancer. In our study, we focused on AC130710, one of lncRNAs. Gastric cancer tissues and adjacent tissues were gathered from 78 patients with gastric cancer. The AC130710 levels were detected by reverse transcription polymerase chain reaction (RT-PCR). Then, we further analyzed the association between AC130710 level and the clinicopathological factors of patients with gastric cancer. Finally, the molecular mechanism underling AC130710 highly expressed in gastric cancer cells was explored. The results showed that AC130710 in cancer tissues from patients with gastric cancer was significantly higher than those in adjacent noncancerous tissues (P < 0.05). Its expression level was significantly associated with tumor size (P = 0.013), tumor-node-metastasis (TNM) stages (P = 0.030), and distal metastasis (P = 0.018). AC130710 expression in MGC-803 was significantly higher than that in normal gastric mucosa cell line GES-1 (P < 0.001). Moreover, miR-129-5p may play an important role in the downregulation of AC130710 in gastric cancer cells. These results indicated that lncRNA-AC130710 may be a potential tumor marker for gastric cancer prognosis.
长链非编码RNA（lncRNAs）在癌症的发生和发展中起着重要作用。然而，lncRNAs在胃癌诊断和治疗中的意义在很大程度上尚不清楚。在我们的研究中，我们聚焦于lncRNAs之一的AC130710。从78例胃癌患者中收集胃癌组织和癌旁组织。通过逆转录聚合酶链反应（RT-PCR）检测AC130710水平。然后，我们进一步分析了AC130710水平与胃癌患者临床病理因素之间的关联。最后，探讨了AC130710在胃癌细胞中高表达的分子机制。结果显示，胃癌患者癌组织中的AC130710显著高于癌旁非癌组织（P < 0.05）。其表达水平与肿瘤大小（P = 0.013）、肿瘤-淋巴结-转移（TNM）分期（P = 0.030）和远处转移（P = 0.018）显著相关。MGC-803中AC130710的表达明显高于正常胃黏膜细胞系GES-1（P < 0.001）。此外，miR-129-5p可能在胃癌细胞中AC130710的下调中起重要作用。这些结果表明，lncRNA-AC130710可能是胃癌预后的潜在肿瘤标志物。
