Department of Infectious Diseases, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Department of Infectious Diseases, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Int J Infect Dis. 2018 Jun;71:107-112. doi: 10.1016/j.ijid.2018.04.798. Epub 2018 Apr 23.
To determine the molecular characteristics and antimicrobial susceptibility of healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in Zhejiang Province.
A total of 391 HA-MRSA isolates were collected from 12 hospitals in five cities of Zhejiang Province, between January 2012 and May 2013. Susceptibility to vancomycin, teicoplanin, linezolid, tigecycline, and daptomycin was determined. Resistant isolates were screened for resistance mutations. Ten isolates from each hospital were then chosen at random for molecular typing.
The isolates showed good susceptibility to all five anti-MRSA agents; only five sporadic non-susceptible isolates were detected. CC5/ST5-MRSA-II-t311 (39/120, 32.5%) was found to be the predominant HA-MRSA clone and was spread between the different hospitals in Hangzhou. CC5/ST5-MRSA-II-t002 was the most prevalent clone in Ningbo, while CC239/ST239-MRSA was epidemic only in certain hospitals in Wenzhou and Shaoxing. Fifteen ST59 isolates (15/120, 12.5%) were identified among the HA-MRSA isolates.
CC5/ST5-MRSA-II-t311 has become the predominant HA-MRSA clone in Hangzhou, Zhejiang Province. ST59 MRSA has spread into hospitals. The isolates showed good susceptibility to all five anti-MRSA agents.
研究浙江省医院获得性耐甲氧西林金黄色葡萄球菌（HA-MRSA）的分子特征和抗菌药物敏感性。
2012 年 1 月至 2013 年 5 月，从浙江省五个城市的 12 家医院采集 391 株 HA-MRSA 分离株。检测万古霉素、替考拉宁、利奈唑胺、替加环素和达托霉素的药敏性。筛选耐药株的耐药突变。然后从每家医院随机选择 10 株进行分子分型。
分离株对五种抗-MRSA 药物均显示出良好的敏感性；仅检测到 5 株散发性非敏感性分离株。CC5/ST5-MRSA-II-t311（39/120，32.5%）是主要的 HA-MRSA 克隆，在杭州的不同医院之间传播。CC5/ST5-MRSA-II-t002 是宁波最流行的克隆，而 CC239/ST239-MRSA 仅在温州和绍兴的某些医院流行。120 株 HA-MRSA 分离株中有 15 株（15/120，12.5%）为 ST59 分离株。
CC5/ST5-MRSA-II-t311 已成为浙江省杭州市主要的 HA-MRSA 克隆。ST59 MRSA 已传播至医院。分离株对五种抗-MRSA 药物均显示出良好的敏感性。
