Kong Haishen, Yu Fei, Zhang Weili, Li Xuefen, Wang Hongxia
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, China.
Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Laboratory Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang UniversityHangzhou, China.
Front Microbiol. 2017 May 12;8:838. doi: 10.3389/fmicb.2017.00838. eCollection 2017.
Analysis of the genotypic characteristics and antimicrobial susceptibility patterns of methicillin-resistant (MRSA) is essential for the control and treatment of diseases caused by this important pathogen. In this study, MRSA isolates obtained from a tertiary caret hospital in China were subjected to typing, SCC typing, multiple locus sequence typing (MLST), and PCR targeting of the genes encoding Panton-Valentine leukocidin (PVL). The disk diffusion method was used to test the antimicrobial susceptibility of the isolates to 10 non-beta-lactam antibiotics. Among the 120 MRSA isolates studied, 18 types and 15 ST types were identified. The t311 type was the most common (a total of 60 isolates; 50%) among the study strains, and nearly all the t311 strains belonged to ST5, which is the most common ST type that was previously reported from China among the t002 isolates. ST5-II/t311 was the major prevalent clone (55, 45.8%), which was followed by ST5-II/t002 (12, 10.0%) and ST59-IV/t437 (11, 9.2%). PVL-encoding genes were found in 6.7% of the isolates. Although the ST5-II/t311 and ST5-II/t002 clones are different types, they shared the same resistance profile (clindamycin, erythromycin, and ciprofloxacin). Most isolates of the ST239-III/t037 clone were resistant to clindamycin, erythromycin, ciprofloxacin, gentamicin, tetracycline, and trimethoprim/sulfamethoxazole. By contrast, the MRSA isolates of the ST239-III/t030 clone were more resistant to rifampin, but they were susceptible to trimethoprim/sulfamethoxazole. Our data emphasize the need for ongoing epidemiologic surveillance.
分析耐甲氧西林金黄色葡萄球菌（MRSA）的基因型特征和抗菌药物敏感性模式对于控制和治疗由这种重要病原体引起的疾病至关重要。在本研究中，从中国一家三级护理医院获得的MRSA分离株进行了分型、葡萄球菌盒式染色体（SCC）分型、多位点序列分型（MLST）以及针对编码杀白细胞素（PVL）基因的PCR检测。采用纸片扩散法检测分离株对10种非β-内酰胺类抗生素的抗菌药物敏感性。在所研究的120株MRSA分离株中，鉴定出18种分型和15种ST型。t311型是研究菌株中最常见的类型（共60株；占50%），几乎所有t311菌株都属于ST5型，这是先前在中国报道的t002分离株中最常见的ST型。ST5-II/t311是主要的流行克隆（55株，占45.8%），其次是ST5-II/t002（12株，占10.0%）和ST59-IV/t437（11株，占9.2%）。在6.7%的分离株中发现了编码PVL的基因。尽管ST5-II/t311和ST5-II/t002克隆是不同的类型，但它们具有相同的耐药谱（对克林霉素、红霉素和环丙沙星耐药）。ST239-III/t037克隆的大多数分离株对克林霉素、红霉素、环丙沙星、庆大霉素、四环素和甲氧苄啶/磺胺甲恶唑耐药。相比之下，ST239-III/t030克隆的MRSA分离株对利福平耐药性更强，但对甲氧苄啶/磺胺甲恶唑敏感。我们的数据强调了持续进行流行病学监测的必要性。
