Pharmacological Sciences Research Lab, Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.
Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan.
BMC Complement Med Ther. 2021 Aug 27;21(1):215. doi: 10.1186/s12906-021-03384-1.
The current study was aimed to investigate the anti-allergic activities of the Umbelliferone (UMB) against the acute Histamine and chronic Picryl chloride (PiCl)-induced allergy in mice. UMB is a coumarin derivative (isolated from Angelica decursiva) found in various parts of the plants such as flowers, roots and, stems isolated from the plants of Umbelliferae family.
The UMB (1, 10, 50 mg/kg) was administered intraperitoneally (i.p) half an h before or 2 h after the induction of allergic ear edema. The acute ear edema was induced by histamine (intradermally, i.d), while the chronic ear edema was induced by painting the PiCl (sensitized with the toluene) on the ear. The antioxidants and oxidative stress markers were assessed. The histological changes were assessed using Hematoxylin and eosin (H and E) and giemsa staining. The immunohistochemistry studies were performed to assess the expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) and inducible nitric oxide synthase (iNOS). The data was analyzed using one-way ANOVA tests followed by Tukey's test with p < 0.05 was chosen as criteria for statistical significance.
UMB treatment markedly reduced the allergic ear edema and ear weight compared to the negative control. Furthermore, the UMB attenuated the oxidative stress markers, while induced the antioxidants enzymes. Similarly, the UMB treatment significantly attenuated the serum immunoglobulin E (IgE) level. The UMB treatment markedly improved the histological parameters using H and E staining and Giemsa staining. The UMB administration induced the Nrf2 expression, while attenuated the iNOS expression. Furthermore, the computational analysis was performed to assess the interaction of the UMB with the various protein targets and to determine the mechanism of interaction with the target proteins.
In conclusion, the UMB treatment significantly alleviated the allergic symptoms, attenuating the oxidative stress, improved the histological features using in vivo and computational approaches.
本研究旨在探讨 Umbelliferone（UMB）对小鼠急性组胺和慢性 Picryl chloride（PiCl）诱导过敏的抗过敏活性。UMB 是一种香豆素衍生物（从当归中分离得到），存在于各种植物部位，如花朵、根部和茎部，分离自伞形科植物。
UMB（1、10、50mg/kg）在诱导过敏耳水肿前半小时或后 2 小时经腹腔（i.p）给药。急性耳水肿由组胺（皮内，i.d）诱导，慢性耳水肿由甲苯敏化的 PiCl（涂于耳上）诱导。评估抗氧化剂和氧化应激标志物。使用苏木精和伊红（H 和 E）和吉姆萨染色评估组织学变化。进行免疫组织化学研究以评估核因子红细胞 2 相关因子 2（Nrf2）和诱导型一氧化氮合酶（iNOS）的表达。使用单因素方差分析（ANOVA）检验分析数据，然后使用 Tukey 检验，选择 p<0.05 作为统计学意义的标准。
与阴性对照组相比，UMB 治疗显著降低了过敏耳水肿和耳重。此外，UMB 减弱了氧化应激标志物，同时诱导了抗氧化酶。同样，UMB 治疗显著降低了血清免疫球蛋白 E（IgE）水平。UMB 治疗通过 H 和 E 染色和吉姆萨染色显著改善了组织学参数。UMB 给药诱导了 Nrf2 表达，同时减弱了 iNOS 表达。此外，进行了计算分析以评估 UMB 与各种蛋白质靶标的相互作用，并确定与靶蛋白相互作用的机制。
总之，UMB 治疗通过体内和计算方法显著减轻过敏症状，减轻氧化应激，改善组织学特征。
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