Cell and Matrix Research Institute, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Department of Dermatology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
J Ethnopharmacol. 2019 Jun 28;238:111850. doi: 10.1016/j.jep.2019.111850. Epub 2019 Apr 4.
Extracts from various parts of Ampelopsis brevipedunculata has been used as anti-inflammatory agents in Asian folk medicine.
To demonstrate the medicinal effect of the A. brevipedunculata in skin inflammation, specifically atopic dermatitis (AD).
The effect of ethanol extract of A. brevipedunculata rhizomes (ABE) on AD was examined using an AD-like skin inflammation model induced by repeated exposure to house dust mite (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene (DNCB). The mechanism study was performed using tumor necrosis factor (TNF)-α and interferon (IFN)-γ-activated human keratinocytes (HaCaT). Serum histamine and immunoglobulin levels were quantified using enzymatic kits, while the gene expression of cytokines and chemokines was analyzed using quantitative real time polymerase chain reaction. The expression of signaling molecules was detected using Western blot.
Oral administration of ABE alleviated DFE/DNCB-induced ear thickening and clinical symptoms, as well as immune cell infiltration (mast cells and eosinophils) into the dermal layer. Serum Immunoglobulin (Ig) E, DFE-specific IgE, IgG2a, and histamine levels were decreased after the administration of ABE. ABE also inhibited CD4IFN-γ and CD4IL-4 lymphocyte polarization in lymph nodes and expression of TNF-α, IFN-γ, IL-4, IL-13, and IL-31 in the ear tissue. In TNF-α/INF-γ-stimulated keratinocytes, ABE inhibited the gene expression of TNF-α, IL-6, IL-1β, and CCL17. In addition, ABE decreased the nuclear localization of signal transducer and activator of transcription 1 and nuclear factor-κB, and the phosphorylation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase.
Collectively, our data demonstrate the pharmacological role and signaling mechanism of ABE in the regulation of skin allergic inflammation, which supports our suggestion that ABE could be developed as a potential therapeutic agent for the treatment of AD.
从各种部分的白首乌已被用作抗炎剂在亚洲民间医学。
为了证明白首乌在皮肤炎症，特别是特应性皮炎（AD）的药用效果。
白首乌根茎乙醇提取物（ABE）对 AD 的影响通过反复暴露于屋尘螨（粉尘螨提取物，DFE）和 2,4-二硝基氯苯（DNCB）诱导的 AD 样皮肤炎症模型进行了检查。使用肿瘤坏死因子（TNF）-α和干扰素（IFN）-γ激活的人角质形成细胞（HaCaT）进行机制研究。使用酶试剂盒定量血清组胺和免疫球蛋白水平，并用定量实时聚合酶链反应分析细胞因子和趋化因子的基因表达。使用 Western blot 检测信号分子的表达。
ABE 的口服给药减轻 DFE/DNCB 诱导的耳朵增厚和临床症状，以及免疫细胞浸润（肥大细胞和嗜酸性粒细胞）到真皮层。ABE 给药后血清免疫球蛋白（Ig）E、DFE 特异性 IgE、IgG2a 和组胺水平降低。ABE 还抑制了淋巴结中 CD4IFN-γ和 CD4IL-4 淋巴细胞的极化以及耳朵组织中 TNF-α、IFN-γ、IL-4、IL-13 和 IL-31 的表达。在 TNF-α/IFN-γ 刺激的角质形成细胞中，ABE 抑制 TNF-α、IL-6、IL-1β 和 CCL17 的基因表达。此外，ABE 减少了信号转导和转录激活因子 1 和核因子-κB 的核定位，以及细胞外信号调节激酶和 p38 丝裂原活化蛋白激酶的磷酸化。
总的来说，我们的数据表明 ABE 在调节皮肤过敏炎症中的药理作用和信号机制，支持我们的建议，即 ABE 可开发为治疗 AD 的潜在治疗剂。
