Singh Pooja, Srivastava Anand Narain, Sharma Rolee, Mateen Saboor, Shukla Bharat, Singh Alok, Chandel Siddhartha
Department of Pathology, Era’s Lucknow Medical College and Hospital, Lucknow. Email:
Asian Pac J Cancer Prev. 2018 Apr 27;19(4):1053-1057. doi: 10.22034/APJCP.2018.19.4.1053.
Background: Circulating miRNAs (miRs) in the biofluids such as serum and plasma act as potential biomarkers for early diagnosis, treatment and prognosis. In the present study, an attempt made to see the expression of miR-21 in serum of 20 cases of Oral sub-mucous fibrosis (OSMF), 20 cases of Oral squamous cell carcinoma and 40 healthy volunteers. The expression of miR-21 was evaluated in relation to different demographical and clinicopathological features such as sex, tobacco, pan-masala, alcohol, smoking and clinical staging respectively with an aim to identify correlation with oral pre-cancer and cancer stages. Materials and Methods: The relative expression level of miR-21 was determined by quantitative real-time RT-PCR (qRT-PCR) in the sera of 20 OSCC, 20 OSMF patients and 40 healthy subjects as a control. Association between expression of miR-21 and OSCC clinical stages and demographical parameters such as sex, pan-masala, tobacco, smoking, alcohol have also been analyzed in detail. Results: The results obtained by t-test revealed significant increase in the expression level of miR-21 in OSCC as compared to OSMF. The study also revealed the positive correlation between higher miR-21 expression and pan-masala chewers as shown by t-test. The statistical test, ANOVA has also indicated a positive correlation between up-regulation of miR-21 in the clinical stages of the OSCC. Conclusion: The results of present study indicated up-regulation of circulating miR-21 in serum of OSCC as compared to OSMF (p=0.001), this study also elucidated the positive correlation between miR-21 expression in OSCC/OSMF patients, only one demographical parameter (Pan-masala) and negative correlation for other parameters such as sex, tobacco, smoking, alcohol etc. Other findings suggested a significant increase (p=0.000) in the expression of miR-21 in clinical staging (I-IV) of oral cancer. More studies are needed to validate it as potential diagnostic and prognostic biomarker for OSMF and OSCC for better management.
血清和血浆等生物流体中的循环微小RNA（miR）可作为早期诊断、治疗和预后的潜在生物标志物。在本研究中，试图观察20例口腔黏膜下纤维化（OSMF）患者、20例口腔鳞状细胞癌患者和40名健康志愿者血清中miR-21的表达情况。分别针对不同的人口统计学和临床病理特征（如性别、烟草、槟榔、酒精、吸烟和临床分期）评估miR-21的表达，旨在确定其与口腔癌前病变和癌症分期的相关性。材料与方法：通过定量实时逆转录聚合酶链反应（qRT-PCR）测定20例口腔鳞状细胞癌（OSCC）患者、20例OSMF患者和40名健康对照者血清中miR-21的相对表达水平。还详细分析了miR-21表达与OSCC临床分期以及人口统计学参数（如性别、槟榔、烟草、吸烟、酒精）之间的关联。结果：t检验结果显示，与OSMF相比，OSCC中miR-21的表达水平显著升高。该研究还通过t检验表明，较高的miR-21表达与嚼槟榔者之间存在正相关。方差分析（ANOVA）统计检验也表明，OSCC临床分期中miR-21的上调之间存在正相关。结论：本研究结果表明，与OSMF相比，OSCC患者血清中循环miR-21上调（p = 0.001），本研究还阐明了OSCC/OSMF患者中miR-21表达与仅一个人口统计学参数（槟榔）之间的正相关，以及与其他参数（如性别、烟草、吸烟、酒精等）之间的负相关。其他研究结果表明，口腔癌临床分期（I-IV期）中miR-21的表达显著增加（p = 0.000）。需要更多研究来验证其作为OSMF和OSCC潜在诊断和预后生物标志物的作用，以实现更好的管理。
